Effect of mechanical toothbrushing combined with different denture cleansers in reducing the viability of a multispecies biofilm on acrylic resins

Author(s)

Dias Panariello, Beatriz Helena ^[1] ; Izumida, Fernanda Emiko ^[2] ; Moffa, Eduardo Buozi ^[2] ; Pavarina, Ana Claudia ^[1] ; Jorge, Janaina Habib ^[1] ; Giampaolo, Eunice Teresinha ^[1]

Affiliation

^[1] Univ Estadual Paulista, Araraquara Dent Sch, Dept Dent Mat & Prosthodont, Rua Humaita 1740, Apto 37, Araraquara, SP - Brazil

^[2] CEUMA Univ, Dept Prosthodont, Sao Luis, Maranhao - Brazil

• Document type: Journal article
• Source: AMERICAN JOURNAL OF DENTISTRY; v. 29, n. 3, p. 154-160, JUN 2016.
• Web of Science Citations: 5

Abstract

Purpose: To investigate the efficacy of immersion and brushing with different cleansing agents in reducing the viability of multispecies biofilm on acrylic resins. Methods: Lucitone 550 (L) and Tokuyama Rebase Fast II (T) specimens (10 x 2 mm) were prepared, sterilized, and inoculated with a suspension of Candida albicans, Candida glabrata, and Streptococcus mutans. Specimens were incubated for 48 hours at 37 degrees C for biofilm formation. Then, they were divided into groups (n=12) and subjected to brushing or immersion for 10 seconds in distilled water (W), 0.2% peracetic acid - Sterilife (Ac), 1% chlorhexidine digluconate (CHX), 1:1 water/dentifrice solution (D), 1% sodium hypochlorite (NaOCl), and sodium perborate/Corega Tabs (Pb). Viable microorganisms were evaluated by the XTT assay and colony counts (cfu/mL). Data were performed by ANOVA and Tukey test with 5% significance level. Results: The multispecies biofilm on L and T were killed by brushing or immersion in Ac, CHX, and NaOCl for only 10 seconds. (AU)

• FAPESP's process: 12/00608-6 - Effectiveness of mechanical brushing with different denture cleansing agents in reducing multi-species biofilm viability and their effects on the surface of acrylic resins
• Grantee: Eunice Teresinha Giampaolo
• Support Opportunities: Regular Research Grants