Advanced search
Start date
Betweenand
Related content
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Susceptibility Profile of Staphylococcus epidermidis and Staphylococcus haemolyticus Isolated from Blood Cultures to Vancomycin and Novel Antimicrobial Drugs over a Period of 12 Years

Full text
Author(s):
Pinheiro, Luiza [1] ; Brito, Carla Ivo [1] ; Pereira, Valeria Cataneli [1] ; Oliveira, Adilson [1] ; Bartolomeu, Ariane Rocha [1] ; Camargo, Carlos Henrique [1, 2] ; Ribeiro Souza Cunha, Maria Lourdes [1]
Total Authors: 7
Affiliation:
[1] Univ Estadual Paulista, UNESP, Inst Biociencias, Dept Microbiol & Imunol, Dist Rubiao Jr S-N, BR-18618970 Botucatu, SP - Brazil
[2] Adolfo Lutz Inst, Ctr Bacteriol, Nucleo Doencas Enter & Infeccoes Patogenos Especi, Sao Paulo, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: MICROBIAL DRUG RESISTANCE; v. 22, n. 4, p. 283-293, JUN 2016.
Web of Science Citations: 10
Abstract

The aim of this study was to evaluate the antimicrobial susceptibility profile of 85 Staphylococcus epidermidis and 84 Staphylococcus haemolyticus strains isolated from blood cultures to oxacillin, vancomycin, tigecycline, linezolid, daptomycin, and quinupristin/dalfopristin over a period of 12 years. S. epidermidis and S. haemolyticus isolated from blood cultures of inpatients, attended at a teaching hospital, were analyzed for the presence of the mecA gene and by SCCmec typing. The minimum inhibitory concentration (MIC) values of tigecycline, linezolid, daptomycin, quinupristin/dalfopristin, and vancomycin were determined. Isolates exhibiting vancomycin MICs of >= 2 mu g/ml were typed by pulsed-field gel electrophoresis (PFGE). The rate of mecA positivity was 92.9% and 100% in S. epidermidis and S. haemolyticus, respectively. The most frequent SCCmec types were type III (53.2%) in S. epidermidis and type I (32.1%) in S. haemolyticus. All isolates were susceptible to linezolid and daptomycin, but 7.1% of S. haemolyticus and 2.3% of S. epidermidis isolates were resistant to tigecycline, and 1.2% each of S. haemolyticus and S. epidermidis were resistant and intermediately resistant to quinupristin/dalfopristin, respectively. S. epidermidis exhibited higher vancomycin MICs (40% with MIC of >= 2 mu g/ml). Clonal typing of strains with vancomycin MIC of >= 2 mu g/ml revealed the presence of different PFGE types of S. epidermidis and S. haemolyticus over a period of up to 4 years (2002-2004, 2005-2008, 2006-2009, 2010-2011). Despite the observation of a high prevalence of mecA, the clinical strains were fully susceptible to vancomycin and to the new drugs linezolid, daptomycin, tigecycline, and quinupristin/dalfopristin. The PFGE types with vancomycin MIC of >= 2 mu g/ml exhibited a great diversity of SCCmec cassettes, demonstrating that S. epidermidis and S. haemolyticus may easily acquire these resistance-conferring genetic elements. (AU)

FAPESP's process: 11/15396-1 - Staphylococcus epidermidis and Staphylococcus haemolyticus: detection of biofilm, toxins genes, antimicrobial resistance and clonal typing in isolates from blood cultures
Grantee:Luiza Hubinger
Support Opportunities: Scholarships in Brazil - Master