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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Global gene expression and morphological alterations in the mammary gland after gestational exposure to bisphenol A, genistein and indole-3-carbinol in female Sprague-Dawley offspring

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Author(s):
Grassi, Tony F. [1] ; da Silva, Glenda N. [2] ; Bidinotto, Lucas T. [3, 4] ; Rossi, Bruna F. [5] ; Quinalha, Marilia M. [5] ; Kass, Laura [6] ; Munoz-de-Toro, Monica [6] ; Barbisan, Luis F. [5]
Total Authors: 8
Affiliation:
[1] Univ Estadual Paulista, UNESP, Botucatu Med Sch, Dept Pathol, BR-18618097 Botucatu, SP - Brazil
[2] Univ Fed Ouro Preto, UFOP, Anal Clin Dept, Ouro Preto, MG - Brazil
[3] Barretos Canc Hosp, Mol Oncol Res Ctr, Barretos, SP - Brazil
[4] Barretos Sch Hlth Sci, Dr Paulo Prata FACISB, Barretos, SP - Brazil
[5] Univ Estadual Paulista, UNESP, Botucatu Biosci Inst, Dept Morphol, BR-18618097 Botucatu, SP - Brazil
[6] Univ Nacl Litoral, Sch Biochem & Biol Sci, Human Pathol Dept, Inst Salud & Ambiente Litoral, CONICET, Santa Fe, NM - Argentina
Total Affiliations: 6
Document type: Journal article
Source: Toxicology and Applied Pharmacology; v. 303, p. 101-109, JUL 15 2016.
Web of Science Citations: 1
Abstract

This study aimed to evaluate the modifying effects of dietary genistein (GEN) and indole-3-carbinol (I3C) on early mammary gland development in female Sprague-Dawley offspring born to mothers exposed to BPA during gestation. Pregnant rats were treated with BPA25 or 250 mu g/kg bw/day from gestational days 10 to 21 with or without dietary intake of GEN (250 mg/kg chow) or I3C (2000 mg/kg chow). At post-natal day (PND) 21, female offspring from different litters were euthanized for mammary gland development and gene expression analyses. Our results indicated that prenatal exposure to BPA25 and 250 did not modify the ductal elongation of the mammary gland tree or the estrogen receptor alpha (ER-alpha) expression in terminal end buds (TEBs). However, BPA25-exposed offspring had a higher number of terminal structures (TEBs + TDs) and an increased mammary branching and cell proliferation index in TEBs. Besides that, BPA25 and 250 modulated the expression of several genes in the immature mammary gland that were not changed in a dose dependent manner and involved different clusters of up- and down-regulated genes. Furthermore, BPA25 and BPA250 + I3C-treated groups also had a higher number of enriched functional gene categories. In addition, maternal dietary GEN and I3C in association with BPA exposure produced specific gene expression alterations in the mammary gland and overcome the adverse effect of BPA25, decreasing the branching of the mammary gland. In conclusion, prenatal BPA exposure induced both morphological and gene expression modifications on the mammary gland that dietary intake of GEN and I3C reverted on BPA25-exposed animals. (C) 2016 Elsevier Inc. All rights reserved. (AU)

FAPESP's process: 11/14655-3 - Evaluation of cell proliferantion, apoptosis and estrogen receptor alpha expression in mammary tissue after in utero exposure to the endocrine disruptor bisphenol A, Indole-3carbinol and genistein in female Sprague-Dawley offsprings.
Grantee:Bruna Fernanda Rossi
Support type: Scholarships in Brazil - Scientific Initiation
FAPESP's process: 11/08523-7 - Chromosomal alterations profiling in brain tumors (medulloblastomas and gliomas): the impact in the identification of new tumorigenic pathways
Grantee:Lucas Tadeu Bidinotto
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 11/06219-9 - Morphometric analysis and validation of digital image analysis of mammary gland differention after in utero exposure to bisfenol A endocrine disrupter and chemopreventive agents on mammary carcinogenesis in Female Sprague-Dawley rats
Grantee:Marília Monteiro Quinalha
Support type: Scholarships in Brazil - Scientific Initiation
FAPESP's process: 09/17894-9 - In Utero endocrine-disrupting bisphenol A exposure and chemopreventive agents on susceptibility to mammary carcinogenesis in female Sprague-Dawley.
Grantee:Tony Fernando Grassi
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 10/14110-4 - In Utero endocrine-disrupting bisphenol A exposure and chemopreventive agents on susceptibility to mammary carcinogenesis in female Sprague-Dawley.
Grantee:Luís Fernando Barbisan
Support type: Regular Research Grants