Rapid and efficient method for the quantification of lychnopholide in rat plasma by liquid chromatography-tandem mass spectrometry for pharmacokinetic application

Author(s)

Lachi-Silva, Larissa ^[1] ; Barreto Sousa, Joao Paulo ^[2] ; Montanha, Maiara Camotti ^[3] ; Sy, Sherwin K. B. ^{[4, 5]} ; Callegari Lopes, Joao Luis ^[2] ; Silva, Denise Brentan ^{[2, 6]} ; Lopes, Norberto Peporine ^[2] ; Diniz, Andrea ^[1] ; Kimura, Elza ^[3]

Affiliation

^[1] Univ Estadual Maringa, Dept Pharm, Preclin Pharmacokinet Lab, Maringa, Parana - Brazil

^[2] Univ Sao Paulo, Nucleo Pesquisa Prod Nat & Sintet, Dept Chem & Phys, Coll Pharmaceut Sci Ribeirao Preto, BR-14049 Ribeirao Preto, SP - Brazil

^[3] Univ Estadual Maringa, Univ Hosp, Nucleo Pesquisa Clin & Bioequivalencia, Maringa, Parana - Brazil

^[4] Univ Florida, Coll Pharm, Dept Pharmaceut, Gainesville, FL 32610 - USA

^[5] Univ Estadual Maringa, Biostat Grad Program, Maringa, Parana - Brazil

^[6] Mato Grosso do Sul Fed Univ, Ctr Biol & Hlth Sci, Lab Prod Nat & Espectrometria Massas, Campo Grande, MS - Brazil

• Document type: Journal article
• Source: BIOMEDICAL CHROMATOGRAPHY; v. 30, n. 7, p. 1092-1096, JUL 2016.
• Web of Science Citations: 1

Abstract

Lychnopholide is a sesquiterpene lactone usually obtained from Lychnophora and Eremanthus species and has pharmacological activities that include anti-inflammatory and anti-tumor. Lychnopholide isolated from Eremanthus matogrossenssis was analyzed in this study. The aims of this study were to develop and validate an analytical methodology by LC-MS/MS and to quantify lychnopholide in rat plasma. Chromatographic separation was achieved on a C-18 column using isocratic elution with the mobile phase consisting of methanol and water (containing 0.1% formic acid) at a flow rate of 0.4mL/min. The detection was performed in multiple-reaction monitoring mode using electrospray ionization in positive mode. The method validation was performed in accordance with regulatory guidelines and the results met the acceptance criteria. The linear range of detection was 10-200ng/mL (r>0.9961). The intra- and inter-day assay variability were <6.2 and <11.7%, respectively. The extraction recovery was approximately 63% using liquid-liquid extraction with chloroform. Lychnopholide was detected in plasma up to 60min after intravenous administration in rats. This rapid and sensitive method for the analysis of the sesquiterpene lactone lychnopholide in rat plasma can be applied to pharmacokinetic studies of this compound. Copyright (c) 2016 John Wiley \& Sons, Ltd. (AU)

• FAPESP's process: 12/18031-7 - Application of MALDI-MS/MS in the vicenin-2 and derivatives analyses: Understanding the ionization processes to production of the images
• Grantee: Denise Brentan da Silva
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 09/54098-6 - Acquisition of a mass spectrometer for the generation of images at the Mass Spectrometry Center of the University of São Paulo Ribeirão Preto School of Pharmaceutical Sciences: studies of molecular localization of biologically active substances
• Grantee: Norberto Peporine Lopes
• Support Opportunities: Multi-user Equipment Program