| Full text | |
| Author(s): Show less - |
Malagrino, Pamella Araujo
[1]
;
Venturini, Gabriela
[1]
;
Yogi, Patricia Schneider
[1]
;
Dariolli, Rafael
[1]
;
Padilha, Kallyandra
[1]
;
Kiers, Bianca
[1]
;
Gois, Tamiris Carneiro
[1]
;
Motta-Leal-Filho, Joaquim Mauricio
[2]
;
Takimura, Celso Kiyochi
[1]
;
Costa Girardi, Adriana Castello
[1]
;
Carnevale, Francisco Cesar
[3]
;
Canevarolo, Rafael
[4]
;
Avancini Costa Malheiros, Denise Maria
;
de Mattos Zeri, Ana Carolina
[4]
;
Krieger, Jose Eduardo
[1]
;
Pereira, Alexandre Costa
[1]
Total Authors: 16
|
| Affiliation: | [1] Univ Sao Paulo, Sch Med, Lab Genet & Mol Cardiol, Inst Heart, Sao Paulo, SP - Brazil
[2] Univ Sao Paulo, Sch Med, Intervent Radiol Unit, Dept Radiol, Heart Inst, Sao Paulo, SP - Brazil
[3] Univ Sao Paulo, Sch Med, Intervent Radiol Unit, Radiol Inst, Hosp Clin, Sao Paulo, SP - Brazil
[4] LNBio, Biosci Natl Lab, Campinas, SP - Brazil
Total Affiliations: 4
|
| Document type: | Journal article |
| Source: | Life Sciences; v. 156, p. 57-67, JUL 1 2016. |
| Web of Science Citations: | 6 |
| Abstract | |
Acute kidney injury (AKI) is a serious complication in hospitalized and transplanted patients, and is mainly caused by ischemia/reperfusion (I/R). However, the current diagnosis of AKI based on acute alterations in serum creatinine or urine output is late and unspecific. To identify new systemic biomarkers for AKI, we performed serum and urine metabolomic profile analyses during percutaneous unilateral renal I/R in a well-controlled swine model. For this, serial serum and urine samples obtained during the pre-ischemia, ischemia and reperfusion periods were analyzed by H-1 nuclear magnetic resonance at 600 MHz. Through the metabolic profiles over I/R, we identified eight serum metabolites that increased with ischemia and recovered to basal values after reperfusion, delineating the ischemic period. In addition, we identified 13 urinary metabolites that changed during the early reperfusion reflecting the ischemic kidney, being able to differentiate between pre-ischemia and post I/R periods. All selected metabolites are described in terms of disease pathophysiology (change of energetic pathway and oxidative stress), which suggest that these serum and urinary metabolites are candidate AKI biomarkers. Interestingly, the selected metabolites allowed us to identify, well described NF kappa B, leptin, INF-gamma and insulin pathways, and a new pathway (Huntingtin) that had not been previously implicated in renal I/R. Huntingtin showed different fragment patterns in ischemic versus non-ischemic kidneys. Therefore, the metabolomic profile found in renal I/R led to the identification of candidate disease biomarkers and a new pathway associated with renal injury. (C) 2016 Elsevier Inc. All rights reserved. (AU) | |
| FAPESP's process: | 12/05447-0 - Heritability of metabolic phenotypes in a Brazilian population |
| Grantee: | Kallyandra Padilha |
| Support Opportunities: | Scholarships in Brazil - Master |
| FAPESP's process: | 13/13526-0 - Identification of protein markers changed by shear stress |
| Grantee: | Gabriela Venturini da Silva |
| Support Opportunities: | Scholarships in Brazil - Doctorate |
| FAPESP's process: | 13/17368-0 - Cardiovascular genomics: mechanisms & novel therapeutics - CVGen mech2ther |
| Grantee: | José Eduardo Krieger |
| Support Opportunities: | Research Projects - Thematic Grants |
| FAPESP's process: | 12/12042-7 - Heritability of Metabolic Phenotypes in the Brazilian Population |
| Grantee: | Alexandre da Costa Pereira |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 11/04344-0 - Identification of nitrated protein with disgnostic potencial and prognostic in desease ischemic kidney |
| Grantee: | Pamella Araujo Malagrino |
| Support Opportunities: | Scholarships in Brazil - Master |