Hyaluronidase decreases neutrophils infiltration to the inflammatory site

Author(s)

Fronza, Marcio ^{[1, 2]} ; Muhr, Cornelia ^[3] ; Calheiros da Silveira, Denise Sayuri ^[1] ; Sorgi, Carlos Arterio ^[1] ; de Paula Rodrigues, Stephen Fernandes ^[4] ; Poliselli Farsky, Sandra Helena ^[4] ; Garcia Paula-Silva, Francisco Wanderley ^[1] ; Merfort, Irmgard ^[3] ; Faccioli, Lucia Helena ^[1]

Affiliation

^[1] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Dept Anal Clin Toxicol & Bromatol, BR-14040903 Ribeirao Preto, SP - Brazil

^[2] Univ Vila Velha, Dept Farm, Vila Velha, Espirito Santo - Brazil

^[3] Univ Freiburg, Dept Pharmaceut Biol & Biotechnol, Hugstetter Str 55, D-79106 Freiburg - Germany

^[4] Univ Sao Paulo, Fac Pharmaceut Sci, Dept Clin & Toxicol Anal, Sao Paulo - Brazil

• Document type: Journal article
• Source: Inflammation Research; v. 65, n. 7, p. 533-542, JUL 2016.
• Web of Science Citations: 6

Abstract

Objective To evaluate the in vivo anti-inflammatory potential of bovine hyaluronidase (HYAL) using two different models of acute inflammation. Methods Air pouches were produced in the dorsal subcutaneous of mice and injected with phosphate saline solution or HYAL. The antiinflammatory action of HYAL was evaluated in carrageenan (Cg)-inflamed air pouches. After 4 and 24 h the cellular influx, protein exudation, cytokines and lipid mediators were evaluated. The action of HYAL on the rolling and adhesion of leukocytes was investigated in the LPS-stimulated mesenteric microcirculation by intravital microscopic. Results Treatment with HYAL reduced the cellular influx and protein exudation in non-inflamed and inflamed air pouches. HYAL treatment of Cg-inflamed air pouch reduced the production of tumor necrosis factor-alpha (TNF-alpha), interleukin-8 (IL-8), leukotriene B4 (LTB4) and LTC4, whereas prostaglandins E-2 (PGE(2)) and D-2 (PGD(2)) concentrations were unchanged. Histological analyses showed that HYAL administration diminished cell infiltration in the air-pouch lining. In LPS-stimulated mesenteric microcirculation, HYAL usage decreased rolling and adhesion of leukocytes, but did not affect the blood vessels diameters. Conclusion The results demonstrate that HYAL inhibited cellular recruitment, edema formation and pro-inflammatory mediators production, resulting in decreased adherence of leukocytes to blood vessels and tissue infiltration. Our data suggest that HYAL may be considered an effective candidate to ameliorate acute inflammation. (AU)

• FAPESP's process: 11/23992-3 - Biological studies of the enzyme hyaluronidase and its possible influence in the skin wound healing process
• Grantee: Marcio Fronza
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 09/07169-5 - Lipid mediators as regulators of immune response
• Grantee: Lúcia Helena Faccioli
• Support Opportunities: Research Projects - Thematic Grants