Fluoride Intensifies Hypercaloric Diet-Induced ER Oxidative Stress and Alters Lipid Metabolism

Author(s)

Barbosa Silva Pereira, Heloisa Aparecida ^[1] ; Dionizio, Aline Salgado ^[2] ; Fernandes, Mileni Silva ^[1] ; Araujo, Tamara Teodoro ^[2] ; Cestari, Tania Mary ^[2] ; Buzalaf, Camila Peres ^[3] ; Iano, Flavia Godoy ^[2] ; Rabelo Buzalaf, Marlia Afonso ^[2]

Affiliation

^[1] Univ Fed Sao Carlos, Dept Genet & Evolut, Ctr Biol Sci & Hlth, Washington Luis, Km 235, BR-13560970 Sao Paulo - Brazil

^[2] Univ Sao Paulo, Dept Biol Sci, Bauru Dent Sch, Al Octavio Pinheiro Brisolla 9-75, BR-17012901 Sao Paulo - Brazil

^[3] Univ Sagrado Coracao, Ctr Ciencias Saude, Rua Irma Arminda 10-50, BR-17011160 Sao Paulo - Brazil

• Document type: Journal article
• Source: PLoS One; v. 11, n. 6 JUN 23 2016.
• Web of Science Citations: 7

Abstract

The role of fluoride (F) in oxidative stress is well reported, but its effects on the lipid metabolism has not been completely explored Background Here, we evaluated the relationship of diet and F-induced oxidative stress to lipid metabolism in the liver of rats eating normocaloric or hypercaloric diets for two time periods (20 or 60 days). Methods Seventy-two 21-day-old Wistar rats were divided into 2 groups (n = 36) based on the type of diet they were eating; each of these groups was then further divided into another two groups (n = 18) based on the time periods of either 20 or 60 days, for a total of four groups. Each of these was divided into 3 subgroups (n = 6 animals/subgroup), dependent on the dose of F administered in the drinking water (0 mg/L(control), 15 mg/L or 50 mg/L). After the experimental period, blood samples and the liver were collected. Plasma samples were analyzed for HDL, cholesterol and triglycerides. Western blots were performed to probe for GRP78, Erp29, SOD2, Apo-E and SREBP in hepatic tissues. Results As expected, the expression of target proteins involved in oxidative stress increased in the F-treated groups, especially in liver tissue obtained from animals eating a hypercaloric diet. Most changes in the lipid levels and pathological conditions were seen earlier in the time period, at day 20. The morphometric analyses showed a reduction in steatosis in groups on ahypercaloric diet and treated with 50 mg F/L compared to the control, while no changes were obtained in normocaloric-fed rats. Accordingly, plasma TG was reduced in the F-treated group. The reduced expression of Apo-E in a time- and diet-dependent pattern may account for the particular decrease in steatosis in hypercaloric-fed F-treated rats. Conclusions These results suggest that F changes liver lipid homeostasis, possibly because of the induction of oxidative stress, which seems to be higher in animals fed hypercaloric diets. (AU)

• FAPESP's process: 11/17263-9 - Effect of treatment time and dose of fluoride chronically administered on protein expression in the liver of rats
• Grantee: Heloisa Aparecida Barbosa da Silva Pereira
• Support Opportunities: Scholarships in Brazil - Doctorate