New Small Plasmid Harboring bla(KPC-2) in Pseudomonas aeruginosa

Author(s)

Galetti, Renata ^[1] ; Andrade, Leonardo Neves ^[1] ; Chandler, Michael ^[2] ; Varani, Alessandro de Mello ^[3] ; Costa Darini, Ana Lucia ^[1]

Affiliation

^[1] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, BR-14049 Ribeirao Preto - Brazil

^[2] CNRS, Lab Microbiol & Genet Mol, Toulouse - France

^[3] Univ Estadual Paulista, Fac Ciencias Agr & Vet, Jaboticabal - Brazil

• Document type: Journal article
• Source: Antimicrobial Agents and Chemotherapy; v. 60, n. 5, p. 3211-3214, MAY 2016.
• Web of Science Citations: 4

Abstract

The aim of this study was to characterize the genetic context of bla(KPC-2) in Pseudomonas aeruginosa sequence type 244 from Brazil. The bla(KPC-2) gene was detected in a new small plasmid, pBH6. Complete sequencing revealed that pBH6 was 3,652 bp long and included the Tn3 resolvase and Tn3 inverted repeat (IR), a partial copy of ISKpn6, and a putative ori region but no rep genes. pBH6 replicated stably into Escherichia coli strain DH10B and P. aeruginosa strain PAO. (AU)

• FAPESP's process: 14/14494-8 - Molecular epidemiology of gram-negative bacteria and genetic of antibiotic resistance
• Grantee: Ana Lúcia da Costa Darini
• Support Opportunities: Research Projects - Thematic Grants