| Full text | |
| Author(s): |
Ersching, Jonatan
[1, 2, 3, 4]
;
Basso, Alexandre Salgado
[2]
;
Garcia Kalich, Vera Lucia
[1]
;
Bortoluci, Karina Ramalho
[5, 3]
;
Rodrigues, Mauricio M.
[2, 3]
Total Authors: 5
|
| Affiliation: | [1] Univ Sao Paulo, Inst Ciencias Biomed, Dept Imunol, Sao Paulo - Brazil
[2] Univ Fed Sao Paulo, Escola Paulista Med, Dept Microbiol Imunol & Parasitol, Sao Paulo - Brazil
[3] Univ Fed Sao Paulo, Escola Paulista Med, CTCMol, Sao Paulo - Brazil
[4] Whitehead Inst Biomed Res, 9 Cambridge Ctr, Cambridge, MA 02142 - USA
[5] Univ Fed Sao Paulo, Dept Ciencias Biol, Sao Paulo - Brazil
Total Affiliations: 5
|
| Document type: | Journal article |
| Source: | PLOS PATHOGENS; v. 12, n. 6 JUN 2016. |
| Web of Science Citations: | 4 |
| Abstract | |
Although CD4(+) Foxp3(+) T cells are largely described in the regulation of CD4(+) T cell responses, their role in the suppression of CD8(+) T cell priming is much less clear. Because the induction of CD8(+) T cells during experimental infection with Trypanosoma cruzi is remarkably delayed and suboptimal, we raised the hypothesis that this protozoan parasite actively induces the regulation of CD8(+) T cell priming. Using an in vivo assay that eliminated multiple variables associated with antigen processing and dendritic cell activation, we found that injection of bone marrow-derived dendritic cells exposed to T. cruzi induced regulatory CD4(+) Foxp3(+) T cells that suppressed the priming of transgenic CD8(+) T cells by peptide-loaded BMDC. This newly described suppressive effect on CD8(+) T cell priming was independent of IL-10, but partially dependent on CTLA-4 and TGF-beta. Accordingly, depletion of Foxp3(+) cells in mice infected with T. cruzi enhanced the response of epitope-specific CD8(+) T cells. Altogether, our data uncover a mechanism by which T. cruzi suppresses CD8(+) T cell responses, an event related to the establishment of chronic infections. (AU) | |
| FAPESP's process: | 12/13032-5 - Generation and analysis of the immunogenicity of recombinant proteins based on the different allelic forms of the circumsporozoite antigen of Plasmodium vivax aiming at the development of a universal vaccine against malaria |
| Grantee: | Irene da Silva Soares |
| Support Opportunities: | Research Projects - Thematic Grants |
| FAPESP's process: | 09/06820-4 - Characterization of antigen presenting cells capable of initiating the immune response and control the immunodominance during Trypanosoma cruzi infection |
| Grantee: | Maurício Martins Rodrigues |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 12/22514-3 - Migration study of specific T cells generated by vaccination or Trypanosoma cruzi infection |
| Grantee: | Jose Ronnie Carvalho de Vasconcelos |
| Support Opportunities: | Research Grants - Young Investigators Grants |