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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Light/Dark Environmental Cycle Imposes a Daily Profile in the Expression of microRNAs in Rat CD133(+) Cells

Full text
Author(s):
Marcola, Marina [1] ; Lopes-Ramos, Camila M. [2] ; Pereira, Eliana P. [1] ; Cecon, Erika [1] ; Fernandes, Pedro A. [1] ; Tamura, Eduardo K. [1] ; Camargo, Anamaria A. [2] ; Parmigiani, Raphael B. [2] ; Markus, Regina P. [1]
Total Authors: 9
Affiliation:
[1] Univ Sao Paulo, Inst Biosci, Lab Chronopharmacol, Dept Physiol, Rua Matao, Travessa 14, 321, Sala 323, Cidade Univ, BR-05508900 Sao Paulo, SP - Brazil
[2] Hosp Sirio Libanes, Ctr Oncol Mol, Sao Paulo, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: Journal of Cellular Physiology; v. 231, n. 9, p. 1953-1963, SEP 2016.
Web of Science Citations: 5
Abstract

The phenotype of primary cells in culture varies according to the donor environmental condition. We recently showed that the time of the day imposes a molecular program linked to the inflammatory response that is heritable in culture. Here we investigated whether microRNAs (miRNAs) would show differential expression according to the time when cells were obtained, namely daytime or nighttime. Cells obtained from explants of cremaster muscle and cultivated until confluence (similar to 20 days) presented high CD133 expression. Global miRNA expression analysis was performed through deep sequencing in order to compare both cultured cells. A total of 504 mature miRNAs were identified, with a specific miRNA signature being associated to the light versus dark phase of a circadian cycle. miR-1249 and miR-129-2-3p were highly expressed in daytime cells, while miR-182, miR-96-5p, miR-146a-3p, miR-146a-5p, and miR-223-3p were highly expressed in nighttime cells. Nighttime cells are regulated for programs involved in cell processes and development, as well as in the inflammation, cell differentiation and maturation; while daytime cells express miRNAs that control stemness and cytoskeleton remodeling. In summary, the time of the day imposes a differential profile regarding to miRNA signature on CD133(+) cells in culture. Understanding this daily profile in the phenotype of cultured cells is highly relevant for clinical outputs, including cellular therapy approaches. (C) 2016 Wiley Periodicals, Inc. (AU)

FAPESP's process: 13/13691-1 - Immune-pineal axis: time-niology integrated to surveillance and defense
Grantee:Regina Pekelmann Markus
Support type: Research Projects - Thematic Grants
FAPESP's process: 11/01304-8 - Effect of melatonin on the subpopulations of cultured endothilial cells
Grantee:Marina Marçola Pereira de Freitas
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 09/17923-9 - Pineal gland as a target for B-amyloid peptide
Grantee:Erika Cecon
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 12/04508-6 - Effect of urban air pollution in São Paulo on pineal gland production of melatonin and on the rat endothelial cell reactivity.
Grantee:Eliana Paula Pereira
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 09/17785-5 - Identification and study of expression of polyadenylation variants and microRNAs by next generation sequencing in samples of colorectal tumor
Grantee:Camila Miranda Lopes Ramos
Support type: Scholarships in Brazil - Doctorate