Effect of long-term ingestion of weakly oxidised flaxseed oil on biomarkers of oxidative stress in LDL-receptor knockout mice

Author(s)

Nogueira, M. S. ^[1] ; Kessuane, M. C. ^[1] ; Lobo Ladd, A. A. B. ^[2] ; Lobo Ladd, F. V. ^[2] ; Cogliati, B. ^[2] ; Castro, I. A. ^[1]

Affiliation

^[1] Univ Sao Paulo, Lab Funct Foods LADAF, Dept Food & Expt Nutr, Fac Pharmaceut Sci, Ave Lineu Prestes 580, B14, BR-05508900 Sao Paulo - Brazil

^[2] Univ Sao Paulo, Sch Vet Med & Anim Sci, Ave Prof Dr Orlando Marques de Paiva 87, BR-05508270 Sao Paulo - Brazil

• Document type: Journal article
• Source: BRITISH JOURNAL OF NUTRITION; v. 116, n. 2, p. 258-269, JUL 28 2016.
• Web of Science Citations: 9

Abstract

The effect of oxidised fatty acids on atherosclerosis progression is controversial. Thus, our objective was to evaluate the effect of long-term consumption of weakly oxidised PUFA from flaxseed oil on oxidative stress biomarkers of LDL-receptor((-/-)) mice. To test our hypothesis, mice were separated into three groups. The first group received a high-fat diet containing fresh flaxseed oil (CONT-), the second was fed the same diet prepared using heated flaxseed oil (OXID), and the third group received the same diet containing fresh flaxseed oil and had diabetes induced by streptozotocin (CONT+). Oxidative stress, aortic parameters and non-alcoholic fatty liver disease were assessed. After 3 months, plasma lipid profile, glucose levels, body weight, energy intake and dietary intake did not differ among groups. Likewise, oxidative stress, plasma malondialdehyde (MDA), hepatic MDA expressed as nmol/mg portion (ptn) and antioxidant enzymes did not differ among the groups. Hepatic linoleic acid, -linolenic acid, arachidonic acid and EPA acid declined in the OXID and CONT+ groups. Aortic wall thickness, lumen and diameter increased only in the OXID group. OXID and CONT+ groups exhibited higher concentrations of MDA, expressed as mol/mg ptn per %PUFA, when compared with the CONT- group. Our results suggest that ingestion of oxidised flaxseed oil increases hepatic MDA concentration and is potentially pro-atherogenic. In addition, the mean MDA value observed in all groups was similar to those reported in other studies that used xenobiotics as oxidative stress inducers. Thus, the diet applied in this study represents an interesting model for further research involving antioxidants. (AU)

• FAPESP's process: 14/18697-0 - Evaluation of oxidized lipids intake in the induction of oxidative stress associated with atherosclerosis in an animal model
• Grantee: Maria Carolina Gomes Kessuane de Arruda
• Support Opportunities: Scholarships in Brazil - Scientific Initiation