Effect of Glutamine, Glutamic Acid and Nucleotides on the Turnover of Carbon (delta C-13) in Organs of Weaned Piglets

Author(s)

Amorim, Alessandro Borges ^[1] ; Berto, Dirlei Antonio ^[2] ; Dib Saleh, Mayra Anton ^[2] ; Telles, Filipe Garcia ^[2] ; Denadai, Juliana Celia ^[2] ; Pereira Sartori, Maria Marcia ^[3] ; Luiggi, Fabiana Golin ^[2] ; Santos, Luan Sousa ^[2] ; Ducatti, Carlos ^[3]

Affiliation

^[1] Univ Fed Mato Grosso, Inst Agr Sci & Technol, Rondonopolis Campus, BR-78735902 Rondonopolis Campus, MG - Brazil

^[2] Sao Paulo State Univ UNESP, Fac Anim Sci & Vet Med, Dept Anim Prod, Botucatu Campus, BR-18618000 Sao Paulo - Brazil

^[3] Sao Paulo State Univ UNESP, Inst Biosci, Environm Stable Isotopes Ctr, BR-18618970 Sao Paulo - Brazil

• Document type: Journal article
• Source: ASIAN-AUSTRALASIAN JOURNAL OF ANIMAL SCIENCES; v. 29, n. 8, p. 1152-1158, AUG 2016.
• Web of Science Citations: 2

Abstract

Morphological and physiological alterations occur in the digestive system of weanling piglets, compromising the performance in subsequent phases. This experiment aimed at verifying the influence of glutamine, glutamate and nucleotides on the carbon turnover in the pancreas and liver of piglets weaned at 21 days of age. Four diets were evaluated: glutamine, glutamic acid or nucleotides-free diet (CD); containing 1% glutamine (GD); containing 1% glutamic acid (GAD) and containing 1% nucleotides (ND). One hundred and twenty-three piglets were utilized with three pigs slaughtered at day zero (weaning day) and three at each one of the experimental days (1, 2, 4, 5, 7, 9, 13, 20, 27, and 49 post-weaning), in order to collect organ samples, which were analyzed for the delta C-13 isotopic composition and compared by means of time. No differences were found (p>0.05) among treatments for the turnover of the C-13 in the pancreas (T-50% = 13.91, 14.37, 11.07, and 9.34 days; T-95% = 46.22, 47.73, 36.79, and 31.04 days for CD, GD, GAD, and ND, respectively). In the liver, the ND presented accelerated values of carbon turnover (T-50% = 7.36 and T-95% = 24.47 days) in relation to the values obtained for the GD (T-50% = 10.15 and T-95% = 33.74 days). However, the values obtained for the CD (T-50% = 9.12 and T-95% = 30.31 days) and GAD (T-50% = 7.83 and T-95% = 26.03 days) had no differences (p>0.05) among other diets. The technique of C-13 isotopic dilution demonstrated trophic action of nucleotides in the liver. (AU)

• FAPESP's process: 11/17844-1 - Effect of glutamine, glutamate and nucleotide on the turnover of carbon ('13C) in body tissues weaners
• Grantee: Dirlei Antonio Berto
• Support Opportunities: Regular Research Grants