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Performance, blood lipid profile and turnover of carbon (´13c) in tissues of weanling pigs fed diets containing nucleotides.

Grant number: 13/17964-2
Support Opportunities:Scholarships in Brazil - Doctorate
Effective date (Start): January 01, 2014
Effective date (End): August 31, 2016
Field of knowledge:Agronomical Sciences - Animal Husbandry - Animal Nutrition and Feeding
Principal Investigator:Dirlei Antonio Berto
Grantee:Gabriela de Mello Miassi
Host Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil


The study aims to evaluate the influence of nucleotide in the cell renewal process and adaptability of piglets at weaning, by studying the turnover of carbon-13 of intestinal mucosa, liver, pancreas and spleen, blood lipid profile and performance. The piglets will be submitted to the feeding program in phases: pre-starter I feed from 21 to 35 days, pre-starter feed II from 36 to 49 days and starter feed from 50 to 70 days old. Following diets will be assessed: control diet without nucleotides (Nu) supplementation, diet containing 0.2% of Nu, diet containing 0.4% of Nu, diet containing 0.6%. In the first experiment will be used 87 weaned piglets distributed in a randomized blocks design with five treatments. On days 3, 5, 8, 12, 20, 32 and 49 after weaning, will be slaughtered three pigs per treatment. On the day of weaning (day zero) will also be slaughtered three pigs. Samples of the intestinal mucosa, liver, pancreas and spleen will be analyzed in a mass spectrometer coupled to a elemental analyzer for determining carbon isotope ratios. In the second experiment will be used 84 weaned piglets in experimental design in randomized blocks with four treatments (levels of nucleotides), seven replicates and three animals per experimental unit to evaluate the average daily feed intake, daily weight gain, conversion feeding and blood lipid profile. In the first experiment, data will be analyzed by the exponential equations method of first order, for comparison between treatments, while in the second experiment will be submitted to variance analysis using the SAS statistical package.

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Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
MIASSI, Gabriela de Mello. Carbon turnover (δ13C) in body tissues and performance of weanling pigs fed diets containing nucleotide. 2016. Doctoral Thesis - Universidade Estadual Paulista (Unesp). Faculdade de Medicina Veterinária e Zootecnia. Botucatu Botucatu.

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