Viral Inhibition Mechanism Mediated by Surface-Modified Silica Nanoparticles

Author(s)

de Souza e Silva, Juliana Martins ^{[1, 2, 3]} ; Melo Hanchuk, Talita Diniz ^{[4, 5, 6]} ; Santos, Murilo Izidoro ^[1] ; Kobarg, Joerg ^{[4, 5, 6]} ; Bajgelman, Marcio Chaim ^[4] ; Cardoso, Mateus Borba ^[1]

Affiliation

^[1] Natl Ctr Res Energy & Mat, Brazilian Synchrotron Light Lab, BR-13083970 Campinas, SP - Brazil

^[2] Tech Univ Munich, Phys Dept, Lehrstuhl Biomed Phys, D-85748 Garching - Germany

^[3] Tech Univ Munich, Inst Med Tech, D-85748 Garching - Germany

^[4] Natl Ctr Res Energy & Mat, Brazilian Biosci Natl Lab, BR-13083970 Campinas, SP - Brazil

^[5] Univ Estadual Campinas, Fac Pharmaceut Sci, BR-13083862 Campinas, SP - Brazil

^[6] Univ Estadual Campinas, Dept Biochem & Tissue Biol, Inst Biol, BR-13083862 Campinas, SP - Brazil

• Document type: Journal article
• Source: ACS APPLIED MATERIALS & INTERFACES; v. 8, n. 26, p. 16564-16572, JUL 6 2016.
• Web of Science Citations: 13

Abstract

Vaccines and therapies are not available for several diseases caused by viruses, thus viral infections result in morbidity and mortality of millions of people every year. Nanoparticles are considered to be potentially effective in inhibiting viral infections. However, critical issues related to their use include their toxicity and their mechanisms of antiviral action, which are not yet completely elucidated. To tackle these problems, we synthesized silica nanoparticles with distinct surface properties and evaluated their biocompatibility and antiviral efficacy. We show that nanoparticles exhibited no significant toxicity to mammalian cells, while declines up to 50% in the viral transduction ability of two distinct recombinant viruses were observed. We designed experiments to address the mechanism of antiviral action of our nanoparticles and found that their hydrophobic/hydrophilic characters play a crucial role. Our results reveal that the use of functionalized silica particles is a promising approach for controlling viral infection and offer promising strategies for viral control. (AU)

• FAPESP's process: 14/22322-2 - Functionalization of silica nanoparticles: increasing biological interaction
• Grantee: Mateus Borba Cardoso
• Support type: Regular Research Grants
• FAPESP's process: 12/05481-4 - Surface chemistry manipulation of silver-based nanocomposites: study of biocidal effect against enveloped virus
• Grantee: Juliana Martins de Souza e Silva
• Support type: Scholarships in Brazil - Post-Doctorate
• FAPESP's process: 13/12190-9 - Understanding the activity of functionalized nanoparticles against virus using coherent diffraction imaging
• Grantee: Juliana Martins de Souza e Silva
• Support type: Scholarships abroad - Research Internship - Post-doctor