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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Molecular identification of Salmonella enterica subsp enterica serovar Gallinarum biovars Gallinarum and Pullorum by a duplex PCR assay

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Author(s):
Alves Batista, Diego Felipe [1] ; de Freitas Neto, Oliveiro Caetano [2] ; de Almeida, Adriana Maria [1] ; Barrow, Paul Andrew [3] ; Barbosa, Fernanda de Oliveira [1] ; Berchieri Junior, Angelo [1]
Total Authors: 6
Affiliation:
[1] Sao Paulo State Univ, Fac Agr & Vet Sci, Sao Paulo - Brazil
[2] Univ Fed Paraiba, Agron Sci Ctr, Campus 2, BR-58397000 Areia, Paraiba - Brazil
[3] Univ Nottingham, Sch Vet Med & Sci, Loughborough, Leics - England
Total Affiliations: 3
Document type: Journal article
Source: JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION; v. 28, n. 4, p. 419-422, JUL 2016.
Web of Science Citations: 4
Abstract

Salmonella enterica subsp. enterica serovar Gallinarum biovar Gallinarum (S. Gallinarum) and biovar Pullorum (S. Pullorum) are 2 poultry pathogens that cause major economic losses to the poultry industry worldwide. Control of both diseases mainly relies on the adoption of biosecurity programs, and success is dependent on accurate and fast detection. Based on this concept, we developed a duplex PCR assay, targeting 2 chromosomal sequences, which allowed us to precisely identify and differentiate S. Gallinarum and S. Pullorum field strains. This assay was validated by testing genomic DNA from 40 S. Gallinarum and 29 S. Pullorum field strains, 87 other Salmonella serovars, and 7 non-Salmonella strains. The serovar identifier region (SIR) primers produced a fragment only in S. Gallinarum and S. Pullorum strains, whereas the fragment from the ratA coding sequence, which was previously demonstrated to differentiate the 2 biovars, was also amplified from other Salmonella serovars. Our results showed that the combination of both SIR and ratA amplifications could be used to identify as well as to differentiate colonies of S. Gallinarum and S. Pullorum reliably. Thus, we believe this methodology can be a useful ancillary tool for routine veterinary diagnostic laboratories by providing rapid, accurate results. (AU)

FAPESP's process: 11/23483-1 - Salmonella Gallinarum (SG): 1. Sequencing of the genome of the attenuate strain SG "cobS" cbiA and assessment of its gene expression in macrophages. 2. Comparative analysis of SG and S. Pullorum genomes. 3. Assessment of virulence of motile SG strains
Grantee:Angelo Berchieri Junior
Support type: Regular Research Grants