| Full text | |
| Author(s): |
Ana Lúcia M. Yamada
[1]
;
Marina L. Alvarenga
[2]
;
Jaqueline S. Brandão
[3]
;
Marcos J. Watanabe
[4]
;
Celso A. Rodrigues
[5]
;
Carlos A. Hussni
[6]
;
Ana L.G. Alves
[7]
Total Authors: 7
|
| Affiliation: | [1] Universidade Estadual Paulista. Faculdade de Medicina Veterinária e Zootecnia. Departamento de Cirurgia e Anestesiologia Veterinária - Brasil
[2] Universidade Estadual Paulista. Faculdade de Medicina Veterinária e Zootecnia. Departamento de Cirurgia e Anestesiologia Veterinária - Brasil
[3] Universidade Estadual Paulista. Faculdade de Medicina Veterinária e Zootecnia. Departamento de Cirurgia e Anestesiologia Veterinária - Brasil
[4] Universidade Estadual Paulista. Faculdade de Medicina Veterinária e Zootecnia. Departamento de Cirurgia e Anestesiologia Veterinária - Brasil
[5] Universidade Estadual Paulista. Faculdade de Medicina Veterinária e Zootecnia. Departamento de Cirurgia e Anestesiologia Veterinária - Brasil
[6] Universidade Estadual Paulista. Faculdade de Medicina Veterinária e Zootecnia. Departamento de Cirurgia e Anestesiologia Veterinária - Brasil
[7] Universidade Estadual Paulista. Faculdade de Medicina Veterinária e Zootecnia. Departamento de Cirurgia e Anestesiologia Veterinária - Brasil
Total Affiliations: 7
|
| Document type: | Journal article |
| Source: | Pesquisa Veterinária Brasileira; v. 36, n. 6, p. 461-467, 2016-06-00. |
| Abstract | |
Abstract: The platelet-rich plasma (PRP) is characterized by its anabolic, anti-inflammatory and gelling capability. Nowadays, the PRP is considered effective in the repair of cartilage defects, and its gelling capability is proper to filling chondral defects. So, the aim of this study was to investigate the use of activated PRP as a fibrin gel scaffold, such as support for the use with mesenchymal stem cells (MSC), on the treatment of experimentally chondral articular defects. Twelve horses were subjected to an arthroscopic surgery at time zero of the experiment (T0). A chondral defect of 15 mm diameter was created on the medial femoral trochlea and these 12 joints were divided into two groups each with six joints in each group (GA and GB). The joints of the GA were treated with implantation of MSC and PRP-gel. GB joints were the control group. MSCs were cultivated from adipose tissue and PRP-gel was obtained by double centrifugation protocol followed by addition of lyophilized thrombin. After five months (T150) was performed new arthroscopy for macroscopic evaluation of the defect local, collect samples of tissue repair for electron microscopy assessment and also was implemented a magnetic resonance images and computed tomography on GA. It was observed that the PRP-gel associated with CTMs showed a suitable treatment of experimental chondral defects in horses. GA showed a better macroscopic and microscopic appearance of the tissue repair. GB showed smaller number of chondrocytes and increased collagen fibers disorganization. At the magnetic resonance and computed tomography imaging only the local of chondral defect was viewed. The PRP-gel scaffold was satisfactory to use and support MSCs implantation. It showed an easy handling and it was effective, showing a promising results in the repair of induced chondral defects. (AU) | |
| FAPESP's process: | 11/19705-9 - MESENCHYMAL STEM CELLS AND PLATELET RICH PLASMA ASSOCIATED WITH MICROFRACTURES ON THE TREATMENT OF ARTICULAR CHONDRAL DEFECTS IN HORSES |
| Grantee: | Ana Lucia Miluzzi Yamada |
| Support Opportunities: | Scholarships in Brazil - Doctorate |
| FAPESP's process: | 12/03600-6 - Mesenchymal stem cells and platelet rich plasma associated with microfractures in the treatment of articular chondral defects in horses |
| Grantee: | Ana Liz Garcia Alves |
| Support Opportunities: | Regular Research Grants |