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(Reference retrieved automatically from SciELO through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Carcinoma ex-pleomorphic adenoma derived from recurrent pleomorphic adenoma shows important difference by array CGH compared to recurrent pleomorphic adenoma without malignant transformation

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Author(s):
Fernanda Viviane Mariano ; Karina Giovanetti ; Luis Fernando Vidal Saccomani ; André Del Negro ; Luiz Paulo Kowalski ; Ana Cristina Victorino Krepischi ; Albina Altemani
Total Authors: 7
Document type: Journal article
Source: Brazilian Journal of Otorhinolaryngology; v. 82, n. 6, p. 687-694, Dez. 2016.
Web of Science Citations: 1
Abstract

Abstract Introduction: A key step of cancer development is the progressive accumulation of genomic changes resulting in disruption of several biological mechanisms. Carcinoma ex-pleomorphic adenoma (CXPA) is an aggressive neoplasm that arises from a pleomorphic adenoma. CXPA derived from a recurrent PA (RPA) has been rarely reported, and the genomic changes associated with these tumors have not yet been studied. Objective: We analyzed CXPA from RPAs and RPAs without malignant transformation using array-comparative genomic hybridization (array-CGH) to identify somatic copy number alterations and affected genes. Methods: DNA samples extracted from FFPE tumors were submitted to array-CGH investigation, and data was analyzed by Nexus Copy Number Discovery Edition v.7. Results: No somatic copy number alterations were found in RPAs without malignant transformation. As for CXPA from RPA, although genomic profiles were unique for each case, we detected some chromosomal regions that appear to be preferentially affected by copy number alterations. The first case of CXPA-RPA (frankly invasive myoepithelial carcinoma) showed copy number alterations affecting 1p36.33p13, 5p and chromosomes 3 and 8. The second case of CXPA-RPA (frankly invasive epithelial-myoepithelial carcinoma) showed several alterations at chromosomes 3, 8, and 16, with two amplifications at 8p12p11.21 and 12q14.3q21.2. The third case of CXPA-RPA (minimally invasive epithelial-myoepithelial carcinoma) exhibited amplifications at 12q13.3q14.1, 12q14.3, and 12q15. Conclusion: The occurrence of gains at chromosomes 3 and 8 and genomic amplifications at 8p and 12q, mainly those encompassing the HMGA2, MDM2, WIF1, WHSC1L1, LIRG3, CDK4 in CXAP from RPA can be a significant promotional factor in malignant transformation. (AU)

FAPESP's process: 11/23366-5 - Genomic profile study of carcinoma ex-pleomorphic adenoma by array-comparative genomic hybridization
Grantee:Fernanda Viviane Mariano Brum Corrêa
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 11/23204-5 - Genomic profile study of carcinoma ex-pleomorphic adenoma by array-comparative genomic hybridization
Grantee:Albina Messias de Almeida Milani Altemani
Support type: Regular Research Grants