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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Cytotoxicity and cytokine expression induced by silorane and methacrylate-based composite resins

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Author(s):
Longo, Daniele Lucca ; Garcia Paula-Silva, Francisco Wanderley ; Faccioli, Lucia Helena ; Gaton-Hernandez, Patricia Maria ; de Queiroz, Alexandra Mussolino ; Bezerra da Silva, Lea Assed
Total Authors: 6
Document type: Journal article
Source: Journal of Applied Oral Science; v. 24, n. 4, p. 338-343, JUL-AUG 2016.
Web of Science Citations: 2
Abstract

ABSTRACT The successful use of composite resins in Dentistry depends on physicochemical properties, but also on the biological compatibility of resins, because of the close association between pulp and dentin. Objective The aim of this study was to evaluate cytotoxicity and cytokine production induced by light-cured or non-light-cured methacrylate-based and silorane composite resins in RAW 264.7 macrophages. Material and Methods Cells were stimulated with the extracts from light-cured or non-light-cured composite resins. After incubation for 24 h, cytotoxicity was assessed with the lactate dehydrogenase (LDH) and methyl thiazolyl tetrazolium (MTT) assays, and total protein was quantified using the Lowry method. TNF-α detection was examined with an enzyme-linked immunosorbent assay (ELISA) conducted with cell supernatants after cell stimulation for 6, 12, and 24 h. Data were analyzed using one-way analysis of variance (ANOVA) and Tukey’s post hoc test (α=0.05). Results KaloreTM and FiltekTM Silorane were cytotoxic with or without light curing (p<0.05) after 24 h of incubation. KaloreTM stimulated the early production of TNF-α in comparison with control (p<0.05), whereas FiltekTM Silorane did not affect TNF-α levels after 6 and 12 h (p>0.05). However, after 24 h FiltekTM Silorane inhibited the production of TNF-α (p<0.05). Conclusions KaloreTM and FiltekTM Silorane were cytotoxic regardless of light curing. The extract obtained from KaloreTM after 15 days of incubation stimulated the production of TNF-α, unlike that obtained from FiltekTM Silorane. (AU)

FAPESP's process: 10/17611-4 - Mechanisms involved in the regulation of 5-lipoxygenase pathway in experimentally-induced apical periodontitis
Grantee:Francisco Wanderley Garcia de Paula e Silva
Support Opportunities: Research Grants - Young Investigators Grants