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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Transmembrane Protein-Based Electrochemical Biosensor for Adiponectin Hormone Quantification

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Author(s):
Brazaca, Lais C. ; Janegitz, Bruno C. ; Cancino-Bernardi, Juliana ; Zucolotto, Valtencir
Total Authors: 4
Document type: Journal article
Source: CHEMELECTROCHEM; v. 3, n. 6, p. 1006-1011, JUN 2016.
Web of Science Citations: 4
Abstract

Decreased adiponectin hormone secretion occurs 10-20 years before chronic hyperglycemia, and is a great indicator of further development of type 2 diabetes. Here, we developed an electrochemical biosensor for adiponectin quantification based on specific transmembrane receptors (AdipoR1 and AdipoR2). Under optimized conditions, cyclic-voltammetry-based adiponectin biosensors displayed linearity (R-2 = 0.992) in a wide range of concentrations (0.025-0.75 mu mol L-1), including typical human serum levels, with a detection limit of 7.0 nmol L-1 and reproducibility of 1.7%, n = 3. Electrochemical impedance spectroscopy results showed a similar behavior, with a linear increase in charge-transfer resistance (R-2 = 0.971) from 0.025 to 1.00 mu mol L-1, a detection limit of 7.0 nmol L-1, and reproducibility of 5.6 %, n = 3. Furthermore, the analytical results obtained by using the biosensor in human serum samples were in agreement with those obtained by using the enzyme-linked immunosorbent assay (ELISA) at a 95% confidence level. It is expected the proposed device increases the number of predictive type2 diabetes diagnostics, promoting early treatment and patient awareness about the precautions to be taken. (AU)

FAPESP's process: 15/19099-2 - Development of electrochemical sensors and biosensors for different analytical purposes
Grantee:Bruno Campos Janegitz
Support Opportunities: Regular Research Grants
FAPESP's process: 13/10608-6 - Development of a system for type 2 diabetes mellitus early diagnosis
Grantee:Laís Canniatti Brazaca
Support Opportunities: Scholarships in Brazil - Master