| Full text | |
| Author(s): |
Renno, C.
;
Nadaf, M. I. V.
;
Zago, C. A.
;
Carneiro-Sampaio, M.
;
Palmeira, P.
Total Authors: 5
|
| Document type: | Journal article |
| Source: | Scandinavian Journal of Immunology; v. 83, n. 6, p. 445-455, JUN 2016. |
| Web of Science Citations: | 3 |
| Abstract | |
Treg cells are crucial to prevent immune dysregulation, but little is known about the frequency of these cells in neonates, particularly in very/moderate and late preterm newborns studied as separate groups. The CD4(+)CD25(hi)CD127(lo)FOXP3(+) Treg population was phenotypically characterized to assess maturation markers and gut-homing integrins by flow cytometry in the cord blood of healthy preterm newborns born at 30-33(6/7) gestation weeks (Group 1), at 34-36(6/7) gestation weeks (Group 2) and term newborns born at 37-41 gestation weeks (Group 3), compared to healthy adults. An inverse correlation of the Treg percentage and gestational age was found, with significantly higher frequencies in Group 1 compared to Groups 2 and 3 and in Group 2 compared to Group 3, and significantly higher Treg frequencies and numbers in the neonates compared to the adults. All of the newborns exhibited increased Treg frequencies with a naive phenotype compared to adults. Cytotoxic T-lymphocyte-associated protein 4 CTLA-4 expression in the naive Treg was decreased in both preterm groups compared with those from term newborns and adults, and in the memory Treg from Group 1 compared with the other groups. The frequencies of Treg expressing alpha 4 beta 7 and alpha 4 beta 1 integrins were higher in both preterm groups, but significantly different only in Group 1, when compared with those from the term newborns and the adults. In conclusion, although a high frequency of Treg is present in newborns, an immature phenotype with a higher expression of CD45RA and alpha 4 beta 7/alpha 4 beta 1 and a lower expression of CTLA-4 is found, particularly in the very preterm group. (AU) | |
| FAPESP's process: | 09/54246-5 - Evaluation of the neonatal immune response: basic mechanisms of activation via Toll-like receptors 2 and 4 (TLR-2 and 4) in different antigen presenting cells from healthy term and preterm newborns and with early and late onset sepsis |
| Grantee: | Patricia Palmeira Daenekas Jorge |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 12/10928-8 - Phenotypic characterization of T regulatory cell population in cord blood of term and preterm newborns |
| Grantee: | Camila Rennó Guimarães |
| Support Opportunities: | Scholarships in Brazil - Master |