Local antibiotic delivery in periodontitis: drug release and its effect on supragingival biofilms

Author(s)

Re, A. C. S. ; Ferreira, M. P. ; Freitas, O. ; Aires, C. P.

• Document type: Journal article
• Source: BIOFOULING; v. 32, n. 9, p. 1061-1066, 2016.
• Web of Science Citations: 4

Abstract

The effect of a drug-delivery system containing antibacterial metronidazole (MDZ) prescribed for periodontitis on supragingival biofilm was evaluated, and possible interference by this biofilm in the drug release profile was investigated. Streptococcus mutans biofilms were grown and exposed to a controlled-release formulation of MDZ or the same formulation without MDZ (vehicle control). Untreated biofilms were used as a negative control (NC). Biofilms and culture medium (containing detached cells) were collected 24, 48, 72, and 96 h after first exposure to treatments. The biomass of the MDZ group was lower than that of the NC group at all times. Although MDZ yielded low drug-release rates in the presence of the biofilm, it was sufficient for reducing viability for 24 h and affecting bacterial metabolism for 48 h. These results suggest that MDZ appears to destabilize supragingival biofilm. This biofilm may interfere with MDZ release from the formulation. (AU)

• FAPESP's process: 12/15556-1 - Suitability and effect of a controlled drug release system in presence of patogenic biofilm
• Grantee: Ana Carolina dos Santos Ré
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 12/15423-1 - Suitability and effect of a controlled drug release system in presence of patogenic biofilm
• Grantee: Carolina Patrícia Aires Garbellini
• Support Opportunities: Regular Research Grants