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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Resistance Training and Ovariectomy: Antagonic Effects in Mitochondrial Biogenesis Markers in Rat Skeletal Muscle

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Barbosa, M. R. ; Shiguemoto, G. E. ; Tomaz, L. M. ; Ferreira, F. C. ; Rodrigues, M. F. C. ; Domingues, M. M. ; Souza Master, M. V. C. ; Canevazzi, G. H. R. ; Silva-Magosso, N. S. ; Selistre-de-Araujo, H. S. ; Perez, E. A.
Total Authors: 11
Document type: Journal article
Source: INTERNATIONAL JOURNAL OF SPORTS MEDICINE; v. 37, n. 11, p. 841-847, OCT 2016.
Web of Science Citations: 1
Abstract

Estrogen reduction is associated with a decline in skeletal muscle mitochondrial biogenesis. Molecular events associated with improvements in markers of mitochondrial biogenesis after resistance training and estradiol replacement are unknown. This study aimed to investigate the effects of ovariectomy, resistance training, and estradiol replacement on markers of mitochondrial biogenesis and protein expression related to oxidative capacity in the rat gastrocnemius pool. Estradiol replacement was performed using Silastic (R) capsules. During the 12-week resistance training, animals climbed a ladder with weights attached to their tails. Gene expression was analysed by RT-PCR, and protein content was determined by western blotting. Ovariectomy decreased the gene expression of the mitochondrial biogenesis markers PGC-1 alpha (similar to 73 %), NRF-1 (similar to 44 %), and TFAM (similar to 53 %) (p < 0.05) and decreased the protein expression of phosphorylated AMPK, CREB and AKT, which are related to oxidative capacity. Resistance training increased PGC-1a (similar to 59 %) and TFAM (similar to 48 %) expression compared to the Ovariectomy-Sedentary group. The combination of resistance training and estradiol replacement was superior to the ovariectomy-sedentary and ovariectomy-resistance training treatments regarding the gastrocnemius muscle. Estrogen deficiency altered the expression of genes and proteins that favour the development of a mitochondrial dysfunction phenotype, which was improved with resistance training and was partially improved by estradiol replacement. (AU)

FAPESP's process: 11/21607-5 - Effects of resistance training in the molecular signalling and tissue remodeling in ovariectomized rats
Grantee:Sergio Eduardo de Andrade Perez
Support Opportunities: Regular Research Grants