Biosynthesis of (-)-ent-kaurenoic acid in Smallanthus sonchifolius and its effect against microbial biofilms

Author(s)

Lopes, Adriana A. ; Pina, Edieidia S. ; Nader, Talita T. ; Da Costa, Fernando B. ; Pereira, Ana Maria S. ; Pupo, Monica T.

• Document type: Journal article
• Source: PHYTOCHEMISTRY LETTERS; v. 18, p. 162-167, DEC 2016.
• Web of Science Citations: 3

Abstract

The biosynthetic pathway of (-)-ent-kaurenoic acid (1) was investigated by incorporation of 1-D-C-13-glucose in Smallanthus sonchifolius (Asteraceae) plantlets. The C-13-enrichment pattern indicated that methylerythritol-4-phosphate (MEP) pathway is the biosynthetic pathway involved in diterpenoid biosynthesis. Our studies in S. sonchifolius reinforce that the biosynthesis of different classes of terpenes should not be compartmentalized into cytosol and plastids. Additionally, (-)-ent-kaurenoic acid showed antimicrobial activity against Staphylococcus aureus biofilm. (C) 2016 Phytochemical Society of Europe. Published by Elsevier Ltd. All rights reserved. (AU)

• FAPESP's process: 13/07600-3 - CIBFar - Center for Innovation in Biodiversity and Drug Discovery
• Grantee: Glaucius Oliva
• Support Opportunities: Research Grants - Research, Innovation and Dissemination Centers - RIDC
• FAPESP's process: 08/01220-6 - Isoprenoid biosynthetic study in Smallanthus sonchifolius and in the endophytic fungus Nigrospora sphaerica associated to the LC-MS monitoring of the sesquiterpene lactone enidrin production in endophytic cultures
• Grantee: Adriana Aparecida Lopes
• Support Opportunities: Scholarships in Brazil - Post-Doctoral