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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Main pathways of action of Brazilian red propolis on the modulation of neutrophils migration in the inflammatory process

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Author(s):
Bueno-Silva, Bruno ; Franchin, Marcelo ; Alves, Claudiney de Freitas ; Denny, Carina ; Colon, David Fernando ; Cunha, Thiago Mattar ; Alencar, Severino Matias ; Napimoga, Marcelo Henrique ; Rosalen, Pedro Luiz
Total Authors: 9
Document type: Journal article
Source: Phytomedicine; v. 23, n. 13, p. 1583-1590, DEC 1 2016.
Web of Science Citations: 19
Abstract

Background: Brazilian propolis is popularly used as treatment for different diseases including the ones with inflammatory origin. Brazilian red propolis chemical profile and its anti-inflammatory properties were recently described however, its mechanism of action has not been investigated yet. Aim: Elucidate Brazilian red propolis major pathways of action on the modulation of neutrophil migration during the inflammatory process. Methods: The ethanolic extract of propolis (EEP) activity was investigated for neutrophil migration into the peritoneal cavity, intravital microscopy (rolling and adhesion of leukocytes), quantification of cytokines TNF-alpha, IL-1 beta and chemokines CXCL1/KC, CXCL2/MIP-2, neutrophil chemotaxis induced by CXCL2/MIP-2, calcium influx and CXCR2 expression on neutrophils. Results: EEP at 10 mg/kg prevented neutrophil migration into peritoneal cavity (p < 0.05), reduced leukocyte rolling and adhesion on the mesenteric microcirculation (p < 0.05) and inhibited the release TNF-alpha, IL-1 beta, CXCL1/KC and CXCL2/MIP-2 (p < 0.05). EEP at 0.01, 0.1 and 1 mu g/ml reduced the CXCL2/MIP-2-induced neutrophils chemotaxis (p < 0.05) without affect cell viability (p > 0.05). EEP at 1 mu g/ml decreased the calcium influx induced by CXCL2/MIP-2 (p < 0.05). On the other hand, none of EEP concentrations tested altered CXCR2 expression by neutrophils (p > 0.05). Conclusion: Brazilian red propolis appears as a promising anti-inflammatory natural product which mechanism seems to be by reducing leukocyte rolling and adhesion; TNF-alpha, IL-1 beta, CXCL1/KC and CXCL2/MIP-2 release; CXCL2/MIP-2-induced chemotaxis and calcium influx. (C) 2016 Elsevier GmbH. All rights reserved. (AU)

FAPESP's process: 12/22378-2 - In vivo and in vitro study of activity of isolated compounds from Brazilian propolis on modulation of neutrophils migration in the inflammatory process
Grantee:Pedro Luiz Rosalen
Support type: Regular Research Grants
FAPESP's process: 12/01365-0 - In vivo and in vitro study of activity of isolated compounds from brazilian propolis on modulation of neutrophils migration in the inflammatory process
Grantee:Marcelo Franchin
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 12/01500-4 - Assessment of effects of propolis and its bioactive compounds on the modulation of of macrophages inflammatory response
Grantee:Bruno Bueno Silva
Support type: Scholarships in Brazil - Post-Doctorate