In vivo and in vitro study of activity of isolated compounds from brazilian propol...
| Full text | |
| Author(s): |
Bueno-Silva, Bruno
;
Franchin, Marcelo
;
Alves, Claudiney de Freitas
;
Denny, Carina
;
Colon, David Fernando
;
Cunha, Thiago Mattar
;
Alencar, Severino Matias
;
Napimoga, Marcelo Henrique
;
Rosalen, Pedro Luiz
Total Authors: 9
|
| Document type: | Journal article |
| Source: | Phytomedicine; v. 23, n. 13, p. 1583-1590, DEC 1 2016. |
| Web of Science Citations: | 19 |
| Abstract | |
Background: Brazilian propolis is popularly used as treatment for different diseases including the ones with inflammatory origin. Brazilian red propolis chemical profile and its anti-inflammatory properties were recently described however, its mechanism of action has not been investigated yet. Aim: Elucidate Brazilian red propolis major pathways of action on the modulation of neutrophil migration during the inflammatory process. Methods: The ethanolic extract of propolis (EEP) activity was investigated for neutrophil migration into the peritoneal cavity, intravital microscopy (rolling and adhesion of leukocytes), quantification of cytokines TNF-alpha, IL-1 beta and chemokines CXCL1/KC, CXCL2/MIP-2, neutrophil chemotaxis induced by CXCL2/MIP-2, calcium influx and CXCR2 expression on neutrophils. Results: EEP at 10 mg/kg prevented neutrophil migration into peritoneal cavity (p < 0.05), reduced leukocyte rolling and adhesion on the mesenteric microcirculation (p < 0.05) and inhibited the release TNF-alpha, IL-1 beta, CXCL1/KC and CXCL2/MIP-2 (p < 0.05). EEP at 0.01, 0.1 and 1 mu g/ml reduced the CXCL2/MIP-2-induced neutrophils chemotaxis (p < 0.05) without affect cell viability (p > 0.05). EEP at 1 mu g/ml decreased the calcium influx induced by CXCL2/MIP-2 (p < 0.05). On the other hand, none of EEP concentrations tested altered CXCR2 expression by neutrophils (p > 0.05). Conclusion: Brazilian red propolis appears as a promising anti-inflammatory natural product which mechanism seems to be by reducing leukocyte rolling and adhesion; TNF-alpha, IL-1 beta, CXCL1/KC and CXCL2/MIP-2 release; CXCL2/MIP-2-induced chemotaxis and calcium influx. (C) 2016 Elsevier GmbH. All rights reserved. (AU) | |
| FAPESP's process: | 12/22378-2 - In vivo and in vitro study of activity of isolated compounds from Brazilian propolis on modulation of neutrophils migration in the inflammatory process |
| Grantee: | Pedro Luiz Rosalen |
| Support type: | Regular Research Grants |
| FAPESP's process: | 12/01365-0 - In vivo and in vitro study of activity of isolated compounds from brazilian propolis on modulation of neutrophils migration in the inflammatory process |
| Grantee: | Marcelo Franchin |
| Support type: | Scholarships in Brazil - Doctorate |
| FAPESP's process: | 12/01500-4 - Assessment of effects of propolis and its bioactive compounds on the modulation of of macrophages inflammatory response |
| Grantee: | Bruno Bueno Silva |
| Support type: | Scholarships in Brazil - Post-Doctorate |