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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Phenolic compounds alone or in combination may be involved in propolis effects on human monocytes

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Author(s):
Cardoso, Eliza de Oliveira ; Conti, Bruno Jose ; Santiago, Karina Basso ; Conte, Fernanda Lopes ; Garcia Oliveira, Lucas Pires ; Hernandes, Rodrigo Tavanelli ; Golim, Marjorie de Assis ; Sforcin, Jose Mauricio
Total Authors: 8
Document type: Journal article
Source: Journal of Pharmacy and Pharmacology; v. 69, n. 1, p. 99-108, JAN 2017.
Web of Science Citations: 4
Abstract

ObjectivesPropolis is a natural product with a complex chemical composition. Its isolated compounds exert biological activities; however, its synergistic effects are unknown. The involvement of phenolic acids (caffeic - Caf, dihydrocinnamic - Cin and p-coumaric - Cou) alone or in combination was investigated in the action of propolis in human monocytes. MethodsCell viability was analysed by MTT assay; TNF-, IL-6 and IL-10 production by enzyme-linked immunosorbent assay (ELISA); cell markers expression by flow cytometry; colony-forming units were counted to assess the microbicidal activity; and H2O2 production was analysed by colorimetric assay. Key findingsTreatments did not affect monocytes viability. Propolis and combinations containing Caf enhanced TNF- production by resting cells. Propolis, Cin, Cou and Caf + Cin stimulated IL-6 production. All treatments upregulated IL-10. In LPS-stimulated cells, treatments downregulated IL-6 and maintained TNF- and IL-10 production. A lower TLR-2 expression was seen than propolis. Caf + Cin enhanced TLR-4 expression. Propolis, Caf and Caf + Cin stimulated H2O2 production, whereas propolis, Cin, Cou, and Caf + Cin + Cou induced a higher fungicidal activity. Cin and Cin + Cou increased the bactericidal activity of human monocytes. ConclusionPropolis activated human monocytes, and acids were involved differently in propolis activity. (AU)

FAPESP's process: 12/14336-8 - Synergistic action of propolis constituents on cytokines production and bactericidal activity of monocytes
Grantee:Eliza de Oliveira Cardoso
Support Opportunities: Scholarships in Brazil - Scientific Initiation
FAPESP's process: 14/13227-6 - A possible synergistic action of propolis components on the activation of human monocytes
Grantee:Eliza de Oliveira Cardoso
Support Opportunities: Scholarships in Brazil - Master