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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Optimization of total RNA isolation from human urinary sediment

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Monteiro, Maria Beatriz ; Santos-Bezerra, Daniele Pereira ; Thieme, Karina ; Passarelli, Marisa ; Machado, Ubiratan Fabres ; Lin, Chin Jia ; Correa-Giannella, Maria Lucia
Total Authors: 7
Document type: Journal article
Source: Clinica Chimica Acta; v. 462, p. 158-161, NOV 1 2016.
Web of Science Citations: 7

Extracting RNA from human urinary sediment is notoriously challenging because of cell paucity and hostile environment and column-based commercial kits using silica technology are commonly used. Nonetheless, in our experience, this methodology yields low amounts of total RNA and has low rates of success. We replaced the column-based commercial kit by a protocol using guanidine isothiocyanate-phenol-chloroform buffer (Trizol reagent) followed by addition of glycogen as a carrier and precipitation with isopropanol plus sodium acetate. This methodology was more affordable and efficient for urinary sediment total RNA isolation than silica technology, resulting in higher concentrations of total RNA of better quality. (C) 2016 Elsevier B.V. All rights reserved. (AU)

FAPESP's process: 15/19000-6 - Study of sediment and microRNAs in the urine of patients with Type 1 diabetes to identify pathways associated to the progression of diabetic kidney disease
Grantee:Maria Beatriz Camargo Monteiro Caillaud
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 12/04831-1 - New players in glycemic control and chronic complications of Diabetes mellitus: preventive and therapeutic perspectives
Grantee:Ubiratan Fabres Machado
Support type: Research Projects - Thematic Grants