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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Layer-by-layer assembly of functionalized reduced graphene oxide for direct electrochemistry and glucose detection

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Tavares Mascagni, Daniela Branco ; Miyazaki, Celina Massumi ; da Cruz, Nilson Cristino ; de Moraes, Marli Leite ; Riul, Jr., Antonio ; Ferreira, Marystela
Total Authors: 6
Document type: Journal article
Source: Materials Science & Engineering C-Materials for Biological Applications; v. 68, p. 739-745, NOV 1 2016.
Web of Science Citations: 14

We report an electrochemical glucose biosensor made with layer-by-layer (LbL) films of functionalized reduced graphene oxide (rGO) and glucose oxidase (GOx). The LbL assembly using positively and negatively charged rGO multilayers represents a simple approach to develop enzymatic biosensors. The electron transport properties of graphene were combined with the specificity provided by the enzyme. rGO was obtained and functionalized using chemical methods, being positively charged with poly(diallyldimethylammonium chloride) to form GPDDA, and negatively charged with poly(styrene sulfonate) to form GPSS. Stable aqueous dispersions of GPDDA and GPSS are easily obtained, enabling the growth of LbL films on various solid supports. The use of graphene in the immobilization of GOx promoted Direct Electron Transfer, which was evaluated by Cyclic Volt-ammetry. Amperometric measurements indicated a detection limit of 13.4 mu mol.L-1 and sensitivity of 2.47 mu mmol(-1).L for glucose with the (GPDDA/GPSS)(1)/(GPDDA/GOx)(2) architecture, whose thickness was 19.80 +/- 0.28 nm, as determined by Surface Plasmon Resonance (SPR). The sensor may be useful for clinical analysis since glucose could be detected even in the presence of typical interfering agents and in real samples of a lactose-free milk and an electrolyte solution to prevent dehydration. (C) 2016 Elsevier B.V. All rights reserved. (AU)

FAPESP's process: 14/15093-7 - Study of nanostructured systems by surface plasmon resonance (SPR): applications in biosensing
Grantee:Celina Massumi Miyazaki
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 14/17519-1 - Immobilization of metal nanoparticules in ultrathin films aiming biosensing
Grantee:Marystela Ferreira
Support type: Regular Research Grants