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(Reference retrieved automatically from SciELO through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

The use of functionalized AFM tips as molecular sensors in the detection of pesticides

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Author(s):
Daiana K. Deda [1] ; Bárbara B. S. Pereira [2] ; Carolina C. Bueno [3] ; Aline N. da Silva [4] ; Gabrielle A. Ribeiro [5] ; Adriano M. Amarante [6] ; Eduardo F. Franca [7] ; Fabio L. Leite [8]
Total Authors: 8
Affiliation:
[1] Federal University of São Carlos. Department of Physics, Chemistry and Mathematics. Laboratory of Nanoneurobiophysics - Brasil
[2] Federal University of São Carlos. Department of Physics, Chemistry and Mathematics. Laboratory of Nanoneurobiophysics - Brasil
[3] Federal University of São Carlos. Department of Physics, Chemistry and Mathematics. Laboratory of Nanoneurobiophysics - Brasil
[4] Federal University of São Carlos. Department of Physics, Chemistry and Mathematics. Laboratory of Nanoneurobiophysics - Brasil
[5] Federal University of São Carlos. Department of Physics, Chemistry and Mathematics. Laboratory of Nanoneurobiophysics - Brasil
[6] Federal University of São Carlos. Department of Physics, Chemistry and Mathematics. Laboratory of Nanoneurobiophysics - Brasil
[7] Federal University of Uberlândia. Institute of Chemistry - Brasil
[8] Federal University of São Carlos. Department of Physics, Chemistry and Mathematics. Laboratory of Nanoneurobiophysics - Brasil
Total Affiliations: 8
Document type: Journal article
Source: MATERIALS RESEARCH-IBERO-AMERICAN JOURNAL OF MATERIALS; v. 16, n. 3, p. 683-687, 2013-03-22.
Abstract

Atomic force spectroscopy, a technique derived from Atomic Force Microscopy (AFM), allowed us to distinguish nonspecific and specific interactions between the acetolactate synthase enzyme (ALS) and anti-atrazine antibody biomolecules and the herbicides imazaquin, metsulfuron-methyl and atrazine. The presence of specific interactions increased the adhesion force (Fadh) between the AFM tip and the herbicides, which made the modified tip a powerful biosensor. Increases of approximately 132% and 145% in the Fadh values were observed when a tip functionalized with ALS was used to detect imazaquin and metsulfuron-methyl, respectively. The presence of specific interactions between the atrazine and the anti-atrazine antibody also caused an increase in the Fadh values (approximately 175%) compared to those observed when using an unfunctionalized tip. The molecular modeling results obtained with the ALS enzyme suggest that the orientation of the biomolecule on the tip surface could be suitable for allowing interaction with the herbicides imazaquin and metsulfuron-methyl. (AU)

FAPESP's process: 09/09120-3 - Beetle luciferases: structural, functional evolution and enegineering for biotechnological purposes
Grantee:Vadim Viviani
Support Opportunities: Regular Research Grants
FAPESP's process: 10/04599-6 - Development of nanobiosensors based on Intelligent surfaces
Grantee:Aline Cristine Nanuh da Silva
Support Opportunities: Scholarships in Brazil - Master
FAPESP's process: 07/05089-9 - Development of nanobiosensors using atomic force spectroscopy: application for detecting pesticides
Grantee:Fabio de Lima Leite
Support Opportunities: Research Grants - Young Investigators Grants