Microsatellite loci in the tiger shark and cross-species amplification using pyrosequencing technology

Author(s):Show less -

Mendes, Natalia J. ; Cruz, Vanessa P. ; Ashikaga, Fernando Y. ; Camargo, Samia M. ; Oliveira, Claudio ; Piercy, Andrew N. ; Burgess, George H. ; Coelho, Rui ; Santos, Miguel N. ; Mendonca, Fernando F. ; Foresti, Fausto

• Document type: Journal article
• Source: PeerJ; v. 4, AUG 30 2016.
• Web of Science Citations: 1

Abstract

The tiger shark (Galeocerdo cuvier) has a global distribution in tropical and warm temperate seas, and it is caught in numerous fisheries worldwide, mainly as bycatch. It is currently assessed as near threatened by the International Union for Conservation of Nature (IUCN) Red List. In this study, we identified nine microsatellite loci through next generation sequencing (454 pyrosequencing) using 29 samples from the western Atlantic. The genetic diversity of these loci were assessed and revealed a total of 48 alleles ranging from 3 to 7 alleles per locus (average of 5.3 alleles). Cross-species amplification was successful at most loci for other species such as Carcharhinus longimanus, C. acronotus and Alopias superciliosus. Given the potential applicability of genetic markers for biological conservation, these data may contribute to the population assessment of this and other species of sharks worldwide. (AU)

• FAPESP's process: 11/23787-0 - Molecular biodiversity and conservation of sharks: gene bank, population structure and international scientific network of collaboration
• Grantee: Fernando Fernandes Mendonça
• Support Opportunities: BIOTA-FAPESP Program - Young Investigators Grants
• FAPESP's process: 13/14555-4 - Development of microsatellite markers for sharks Carcharhinus acronotus and Galeocerdo cuvier using second generation sequencing
• Grantee: Natália Jade Mendes
• Support Opportunities: Scholarships in Brazil - Master