Development and characterization of microsatellite loci for the Neotropical orchid Trichocentrum pumilum

Author(s)

Orth Ritter Antiqueira, Lia Maris ; Dequigiovanni, Gabriel ; Tambarussi, Evandro Vagner ; Rodrigues, Jucelene Fernandes ; Veasey, Elizabeth Ann

• Document type: Journal article
• Source: Crop Breeding and Applied Biotechnology; v. 16, n. 4, p. 355-360, OCT-DEC 2016.
• Web of Science Citations: 0

Abstract

Studies of genetic diversity and structure are key elements in designing effective in situ and ex situ management plans, especially for species experiencing forest fragmentation. To investigate the level of genetic diversity in populations of Trichocentrum pumilum, eight polymorphic microsatellite loci were developed and used for genotyping 96 specimens from four disturbed populations. Low genetic diversity within populations was found (average number of alleles per locus ranging from 3.75 to 4.25, observed and expected heterozygosities from 0.238 to 0.333 and from 0.450 to 0.482, respectively). The fixation index (F-IS) ranged from 0.35 to 0.47, with significant values for all populations. No genotypic disequilibrium was detected. A mixed breeding system was found through an apparent outcrossing rate estimate. Our results suggest that these microsatellite loci are suitable for genetic studies of this species, showing low within population genetic diversity and moderate structure for T. pumilum populations. (AU)

• FAPESP's process: 13/13918-6 - Spatial genetic structure and diversity in fine-scale of Trichocentrum pumilum (Lindl.) M. W. Chase & N. H. Williams estimated by microsatellite markers
• Grantee: Lia Maris Orth Ritter Antiqueira
• Support Opportunities: Scholarships in Brazil - Post-Doctoral