Zika virus disrupts molecular fingerprinting of human neurospheres

Author(s):Show less -

Garcez, Patricia P. ; Nascimento, Juliana Minardi ; de Vasconcelos, Janaina Mota ; da Costa, Rodrigo Madeiro ; Delvecchio, Rodrigo ; Trindade, Pablo ; Loiola, Erick Correia ; Higa, Luiza M. ; Cassoli, Juliana S. ; Vitoria, Gabriela ; Sequeira, Patricia C. ; Sochacki, Jaroslaw ; Aguiar, Renato S. ; Fuzii, Hellen Thais ; Bispo de Filippis, Ana M. ; Goncalves Vianez Junior, Joao Lidio da Silva ; Tanuri, Amilcar ; Martins-de-Souza, Daniel ; Rehen, Stevens K.

• Document type: Journal article
• Source: SCIENTIFIC REPORTS; v. 7, JAN 23 2017.
• Web of Science Citations: 34

Abstract

Zika virus (ZIKV) has been associated with microcephaly and other brain abnormalities; however, the molecular consequences of ZIKV to human brain development are still not fully understood. Here we describe alterations in human neurospheres derived from induced pluripotent stem (iPS) cells infected with the strain of Zika virus that is circulating in Brazil. Combining proteomics and mRNA transcriptional profiling, over 500 proteins and genes associated with the Brazilian ZIKV infection were found to be differentially expressed. These genes and proteins provide an interactome map, which indicates that ZIKV controls the expression of RNA processing bodies, miRNA biogenesis and splicing factors required for self-replication. It also suggests that impairments in the molecular pathways underpinning cell cycle and neuronal differentiation are caused by ZIKV. These results point to biological mechanisms implicated in brain malformations, which are important to further the understanding of ZIKV infection and can be exploited as therapeutic potential targets to mitigate it. (AU)

• FAPESP's process: 14/21035-0 - Quantitative proteomics in neural cell lines and organoids derived from induced pluripotent stem cells from schizophrenia patients
• Grantee: Juliana Minardi Nascimento
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 14/10068-4 - Multi-User Equipment approved in grant 13/08711-3: mass spectrometer waters SYNAPT G2-Si HDMS + nanoACQUITY UPLC
• Grantee: Daniel Martins-de-Souza
• Support Opportunities: Multi-user Equipment Program
• FAPESP's process: 13/08711-3 - Developing a predictive test for a successful medication response and understanding the molecular bases of schizophrenia through proteomics
• Grantee: Daniel Martins-de-Souza
• Support Opportunities: Research Grants - Young Investigators Grants
• FAPESP's process: 14/14881-1 - Understanding the influence of glycolysis components in the function of oligodendrocytes: linking with findings in schizophrenia
• Grantee: Juliana Silva Cassoli
• Support Opportunities: Scholarships in Brazil - Post-Doctoral