Label-free potentiometric biosensor based on solid-contact for determination of total phenols in honey and propolis

Author(s)

Draghi, Patricia Ferrante ; Bastos Fernandes, Julio Cesar

• Document type: Journal article
• Source: Talanta; v. 164, p. 413-417, MAR 1 2017.
• Web of Science Citations: 9

Abstract

We developed a label-free potentiometric biosensor using tyrosinase extracted from Musa acuminata and immobilized by covalent bond on a surface of a solid-contact transducer. The transducer was manufactured containing two layers. The first layer contained a blend of poly(vinyl) chloride carboxylated (PVC-COOH), graphite and potassium permanganate. On this layer, we deposited a second layer containing just a mixture of poly(vinyl chloride) carboxylated and graphite. On the last layer of the transducer, we immobilized the tyrosinase enzyme by reaction with N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride. The solid-contact potentiometric biosensor presented at low detection limit of 7.3x10(-7) M and a linear range to catechol concentration between 9.3x10(-7) M and 8.3x10(-2) M. This biosensor was applied to determine the amount of total phenols in different samples of honey and propolis. The results agreed with the Folin-Ciocalteu method. (AU)

• FAPESP's process: 15/18299-8 - Development of a potentiometric biosensor for determination of polyphenols in honey
• Grantee: Patricia Ferrante Draghi
• Support Opportunities: Scholarships in Brazil - Scientific Initiation