Laboratory strains of Aedes aegypti are competent to Brazilian Zika virus

Author(s)

Costa-da-Silva, Andre Luis ; Ioshino, Rafaella Sayuri ; Correa de Araujo, Helena Rocha ; Kojin, Bianca Burini ; de Andrade Zanotto, Paolo Marinho ; Leal Oliveira, Danielle Bruna ; Melo, Stella Rezende ; Durigon, Edison Luiz ; Capurro, Margareth Lara

• Document type: Journal article
• Source: PLoS One; v. 12, n. 2 FEB 10 2017.
• Web of Science Citations: 17

Abstract

The Zika virus outbreaks are unprecedented human threat in relation to congenital malformations and neurological/autoimmune complications. Since this virus has high potential to spread in regions presenting the vectors, improvement in mosquito control is a top priority. Thus, Aedes aegypti laboratory strains will be fundamental to support studies in different research fields implicated on Zika-mosquito interactions which are the basis for the development of innovative control methods. In this sense, our aim was to determine the main infection aspects of a Brazilian Zika strain in reference Aedes aegypti laboratory mosquitoes. We orally exposed Rockefeller, Higgs and Rexville mosquitoes to the Brazilian ZIKV (ZIKV(BR)) and qRT-PCR was applied to determine the infection, dissemination and detection rates of ZIKV in the collected saliva as well as viral levels in mosquito tissues. The three strains sustain the virus development but Higgs showed significantly lower viral loads in bodies at 14 days post-infection (dpi) and the lowest prevalences in bodies and heads. The Rockefeller strain was the most susceptible at 7 dpi but similar dissemination rates were observed at 14 dpi. Although variations exist, the ZIKVBR RNA shows detectable levels in saliva of the three strains at 14 dpi but is only detected in Rockefeller at 7 dpi. Moreover, saliva samples from the three strains were confirmed to be infectious when intrathoracically injected into mosquitoes. The ZIKVBR kinetics was monitored in Rockefeller mosquitoes and virus could be identified in the heads at 4 dpi but was more consistently detected late in infection. Our study presents the first evaluation on how Brazilian Zika virus behaves in reference Aedes aegypti strains and shed light on how the infection evolves over time. Vector competence and hallmarks of the ZIKVBR development were revealed in laboratory mosquitoes, providing additional information to accelerate studies focused on ZIKV-mosquito interactions. (AU)

• FAPESP's process: 13/19921-9 - Improvement and evaluation of Aedes aegypti transgenic lines to control dengue transmission
• Grantee: Margareth de Lara Capurro-Guimarães
• Support Opportunities: Regular Research Grants
• FAPESP's process: 14/17766-9 - A systemic approach to study permissivity on the Anticarsia gemmatalis multiple nucleopolyhedrovirus (AgMNPV)
• Grantee: Paolo Marinho de Andrade Zanotto
• Support Opportunities: Regular Research Grants
• FAPESP's process: 13/16609-4 - Aedes aegypti sex conversion by transgenesis
• Grantee: Helena Corrêa de Araújo Gomes
• Support Opportunities: Scholarships in Brazil - Post-Doctoral