The molecular motor Myosin Va interacts with the cilia-centrosomal protein RPGRIP1L

Author(s):Show less -

Assis, L. H. P. ; Silva-Junior, R. M. P. ; Dolce, L. G. ; Alborghetti, M. R. ; Honorato, R. V. ; Nascimento, A. F. Z. ; Melo-Hanchuk, T. D. ; Trindade, D. M. ; Tonoli, C. C. C. ; Santos, C. T. ; Oliveira, P. S. L. ; Larson, R. E. ; Kobarg, J. ; Espreafico, E. M. ; Giuseppe, P. O. ; Murakami, M. T.

• Document type: Journal article
• Source: SCIENTIFIC REPORTS; v. 7, MAR 7 2017.
• Web of Science Citations: 15

Abstract

Myosin Va (MyoVa) is an actin-based molecular motor abundantly found at the centrosome. However, the role of MyoVa at this organelle has been elusive due to the lack of evidence on interacting partners or functional data. Herein, we combined yeast two-hybrid screen, biochemical studies and cellular assays to demonstrate that MyoVa interacts with RPGRIP1L, a cilia-centrosomal protein that controls ciliary signaling and positioning. MyoVa binds to the C2 domains of RPGRIP1L via residues located near or in the Rab11a-binding site, a conserved site in the globular tail domain (GTD) from class V myosins. According to proximity ligation assays, MyoVa and RPGRIP1L can interact near the cilium base in ciliated RPE cells. Furthermore, we showed that RPE cells expressing dominant-negative constructs of MyoVa are mostly unciliated, providing the first experimental evidence about a possible link between this molecular motor and cilia-related processes. (AU)

• FAPESP's process: 14/09720-9 - Studies of the role of phosphorylation and protein binding partners in regulating the function of human class V myosins
• Grantee: Mário Tyago Murakami
• Support Opportunities: Regular Research Grants
• FAPESP's process: 14/18189-5 - Studying a possible functional circuitry involving microRNAs, MITF, MYO5A/DLC2, RAB27A and connections with the invasion and metastasis cascade
• Grantee: Enilza Maria Espreafico
• Support Opportunities: Regular Research Grants
• FAPESP's process: 09/54014-7 - Acquisition of a biophotonic imaging system and a multiphoton microscopy system for in vivo imaging
• Grantee: Enilza Maria Espreafico
• Support Opportunities: Multi-user Equipment Program
• FAPESP's process: 11/20229-7 - Human myosin-5A and the intracellular transport of cargoes: studies of the myosin-5A and adapter proteins complexes
• Grantee: Leandro Henrique de Paula Assis
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• FAPESP's process: 09/08312-6 - Functional and structural investigations of the molecular mechanisms involved in signaling, regulation and activation of Class V myosins
• Grantee: Mário Tyago Murakami
• Support Opportunities: Regular Research Grants