Multimodal evaluation of ultra-short laser pulses treatment for skin burn injuries

Author(s)

dos Santos, Moises Oliveira ; Latrive, Anne ; Augusto de Castro, Pedro Arthur ; de Rossi, Wagner ; Tenorio Zorn, Telma Maria ; Samad, Ricardo Elgul ; Freitas, Anderson Zanardi ; Cesar, Carlos Lenz ; Vieira Junior, Nilson Dias ; Zezell, Denise Maria

• Document type: Journal article
• Source: BIOMEDICAL OPTICS EXPRESS; v. 8, n. 3, p. 1575-1588, MAR 1 2017.
• Web of Science Citations: 3

Abstract

Thousands of people die every year from burn injuries. The aim of this study is to evaluate the feasibility of high intensity femtosecond lasers as an auxiliary treatment of skin burns. We used an in vivo animal model and monitored the healing process using 4 different imaging modalities: histology, Optical Coherence Tomography (OCT), Second Harmonic Generation (SHG), and Fourier Transform Infrared (FTIR) spectroscopy. 3 dorsal areas of 20 anesthetized Wistar rats were burned by water vapor exposure and subsequently treated either by classical surgical debridement, by laser ablation, or left without treatment. Skin burn tissues were non-invasively characterized by OCT images and biopsied for further histopathology analysis, SHG imaging and FTIR spectroscopy at 3, 5, 7 and 14 days after burn. The laser protocol was found as efficient as the classical treatment for promoting the healing process. The study concludes to the validation of femtosecond ultra-short pulses laser treatment for skinburns, with the advantage of minimizing operatory trauma. (C) 2017 Optical Society of America (AU)

• FAPESP's process: 05/51689-2 - Optics and Photonics Research Center at UNICAMP
• Grantee: Hugo Luis Fragnito
• Support Opportunities: Research Grants - Research, Innovation and Dissemination Centers - RIDC
• FAPESP's process: 11/51959-0 - Biology of neoplastic diseases of bone marrow
• Grantee: Sara Teresinha Olalla Saad
• Support Opportunities: Research Projects - Thematic Grants