Frequency of Application of AmF/NaF/SnCl2 Solution and Its Potential in Controlling Human Enamel Erosion Progression: An in situ Study

Author(s)

da Silva, C. V. ; Ramos-Oliveira, T. M. ; Mantilla, T. F. ; de Freitas, P. M.

• Document type: Journal article
• Source: Caries Research; v. 51, n. 2, p. 141-148, 2017.
• Web of Science Citations: 4

Abstract

Although several studies have demonstrated the efficacy of AmF/NaF/SnCl2 solution in inhibiting dental erosion progression, measures for further improvement in its effectiveness are paramount. Thus, this in situ study evaluated whether the protective effect promoted by the AmF/NaF/SnCl2 solution would be enhanced by increasing its frequency of use. The study was conducted with 12 volunteers, a 4-phase (5 days each) randomized, crossover model. Extraoral erosive challenges (0.5% citric acid, pH 2.6, 6 x 2 min/day) and rinsing protocol (1 or 2 x 2 min/day) were performed. Before the in situ phase, human enamel samples were subjected to an in vitro surface softening (1% citric acid, pH 4.0, for 3 min). Four treatment protocols were tested using samples in replicas (n = 12): group G1 - deionized water (negative control); G2 - NaF solution (positive control, 500 ppm F-, pH 4.5); G3 - AmF/NaF/SnCl2 solution (500 ppm F-, 800 ppm Sn2+, pH 4.5) once a day; G4 - AmF/NaF/SnCl2 solution twice a day. Tissue loss and morphological changes were determined by optical profilometry (n = 12) and scanning electron microscopy (n = 3) analysis, respectively. Data were statistically analyzed by ANOVA with subsequent pairwise comparison of treatments. Tissue loss means (+/- SD in pm) for each treatment protocol and statistical differences were found as follows: G1 4.55 +/- 2.75, G2 4.59 +/- 2.13, G3 2.64 +/- 1.55, and G4 1.34 +/- 1.16. Although there was no difference between the 2 AmF/NaF/SnCl2 solution application regimens (once or twice a day), application of the product twice a day was the only treatment that was able to control erosion progression, differing from the control groups. (C) 2017 S. Karger AG, Basel (AU)

• FAPESP's process: 14/10955-0 - In situ evaluation of the SnF2 potential in inhibiting the progression of the erosion process on human dental enamel
• Grantee: Camila Vieira da Silva
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 15/10248-5 - Evaluation of the TiF4 gel protector effect on eroded human dentin: an in situ study
• Grantee: Patricia Moreira de Freitas Costa e Silva
• Support Opportunities: Regular Research Grants