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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Deletion of pH Regulator pac-3 Affects Cellulase and Xylanase Activity during Sugarcane Bagasse Degradation by Neurospora crassa

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Author(s):
Pedersoli, Wellington Ramos ; Santos, Rodrigo da Silva [1] ; Vieira Nogueira, Karoline Maria [2] ; Silva-Rocha, Rafael ; Rossi, Antonio [8]
Total Authors: 5
Affiliation:
[1] Silva, Roberto Nascimento, Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Biochem \& Immunol, Ribeirao Preto, SP, Brazil. Pedersoli, Wellington Ramos, Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Genet, Ribeirao Preto, SP, Brazil. Silva-Rocha, Rafael, Univ - Brazil
[2] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Genet, Ribeirao Preto, SP, Brazil. Silva-Rocha, Rafael, Univ Sao Paulo, Ribeirao Preto Med Sch, Syst & Synthet Biol Lab, Dept Cell & Mol Biol, Ribeirao Preto, SP - Brazil
[3] Silva, Roberto Nascimento, Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Biochem \& Immunol, Ribeirao Preto, SP, Brazil. Pedersoli, Wellington Ramos, Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Genet, Ribeirao Preto, SP, Brazil. Silva-Rocha, Rafael, Univ Sao Paulo, Ribeirao Preto Med Sch, Syst & Synthet Biol Lab, Dept Cell & Mol Biol, Ribeirao Preto, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: PLoS One; v. 12, n. 1 JAN 20 2017.
Web of Science Citations: 8
Abstract

Microorganisms play a vital role in bioethanol production whose usage as fuel energy is increasing worldwide. The filamentous fungus Neurospora crassa synthesize and secrete the major enzymes involved in plant cell wall deconstruction. The production of cellulases and hemicellulases is known to be affected by the environmental pH; however, the regulatory mechanisms of this process are still poorly understood. In this study, we investigated the role of the pH regulator PAC-3 in N. crassa during their growth on sugarcane bagasse at different pH conditions. Our data indicate that secretion of cellulolytic enzymes is reduced in the mutant Delta pac-3 at alkaline pH, whereas xylanases are positively regulated by PAC-3 in acidic (pH 5.0), neutral (pH 7.0), and alkaline (pH 10.0) medium. Gene expression profiles, evaluated by real-time qPCR, revealed that genes encoding cellulases and hemicellulases are also subject to PAC-3 control. Moreover, deletion of pac-3 affects the expression of transcription factor-encoding genes. Together, the results suggest that the regulation of holocellulase genes by PAC-3 can occur as directly as in indirect manner. Our study helps improve the understanding of holocellulolytic performance in response to PAC-3 and should thereby contribute to the better use of N. crassa in the biotechnology industry. (AU)

FAPESP's process: 14/09493-2 - Identification and characterization of novel transcription factors involved in the regulation of lignocellulosic biomass degradation in Trichoderma reesei (Hypocrea jecorina)
Grantee:Amanda Cristina Campos Antoniêto
Support Opportunities: Scholarships in Brazil - Doctorate
FAPESP's process: 14/03847-7 - Molecular characterization of mechanisms involved in pathogenicity and cell signaling in fungi
Grantee:Nilce Maria Martinez-Rossi
Support Opportunities: Research Projects - Thematic Grants