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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Temporal Profile of Biofilm Formation, Gene Expression and Virulence Analysis in Candida albicans Strains

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Author(s):
de Barros, Patricia Pimentel ; Rossoni, Rodnei Dennis ; Ribeiro, Felipe De Camargo ; Junqueira, Juliana Campos ; Cardoso Jorge, Antonio Olavo
Total Authors: 5
Document type: Journal article
Source: Mycopathologia; v. 182, n. 3-4, p. 285-295, APR 2017.
Web of Science Citations: 5
Abstract

The characterization of Candida albicans strains with different degrees of virulence became very useful to understand the mechanisms of fungal virulence. Then, the objective of this study was to assess and compare the temporal profiles of biofilms formation, gene expression of ALS1, ALS3, HWP1, BCR1, EFG1, TEC1, SAP5, PLB2 and LIP9 and virulence in Galleria mellonella of C. albicans ATCC18804 and a clinical sample isolated from an HIV-positive patient (CA60). Although the CFU/mL counting was higher in biofilms formed in vitro by ATCC strain, the temporal profile of the analysis of the transcripts of the C. albicans strains was elevated to Ca60 compared to strain ATCC, especially in the genes HWP1, ALS3, SAP5, PLB2 and LIP9 (up regulation). Ca60 was more pathogenic for G. mellonella in the survival assay (p = 0.0394) and hemocytes density (p = 0.0349), agreeing with upregulated genes that encode the expression of hyphae and hydrolase genes of Ca60. In conclusion, the C. albicans strains used in this study differ in the amount of biofilm formation, virulence in vivo and transcriptional profiles of genes analyzed that can change factors associated with colonization, proliferation and survival of C. albicans at different niches. SAP5 and HWP1 were the genes more expressed in the formation of biofilm in vitro. (AU)

FAPESP's process: 12/15250-0 - EVALUATION OF TEMPORAL EXPRESSION OF GENES SAP5, SAP9, PLB1, PLB2, LIP9 LIP10 IN INTERACTIONS BETWEEN SPECIES C. albicans, C. tropicalis, C. glabrata and C. krusei.
Grantee:Patrícia Pimentel de Barros
Support Opportunities: Scholarships in Brazil - Doctorate
FAPESP's process: 12/02184-9 - Interactions between different Candida species in experimental candidiasis of invertebrates and vertebrates models
Grantee:Juliana Campos Junqueira
Support Opportunities: Regular Research Grants
FAPESP's process: 11/15194-0 - Interactions between different Candida species in experimental candidiasis of invertebrates and vertebrates models
Grantee:Rodnei Dennis Rossoni
Support Opportunities: Scholarships in Brazil - Master