Type 2 Diabetes Remission After Roux-en-Y Gastric Bypass: Evidence for Increased Expression of Jejunal Genes Encoding Regenerating Pancreatic Islet-Derived Proteins as a Potential Mechanism

Author(s)

Sala, Priscila ; Torrinhas, Raquel Susana ; Fonseca, Danielle Cristina ; Heymsfield, Steven ; Giannella-Neto, Daniel ; Waitzberg, Dan Linetzky

• Document type: Journal article
• Source: OBESITY SURGERY; v. 27, n. 4, p. 1123-1127, APR 2017.
• Web of Science Citations: 8

Abstract

Background Intestinal expression of regenerating pancreatic islet-derived protein-encoding genes (REG) would be enhanced after Roux-en-Y gastric bypass (RYGB) and would affect postoperative type 2 diabetes remission (T2Dr). Methods Intestinal biopsy samples were collected from 20 adult obese women with T2D before and 3 months after RYGB. Levels of REG expression and the gene encoding its putative receptor (EXTL3) were assessed by microarray and validated by quantitative RT-PCR. T2Dr was assessed according to ADA criteria 1 year after RYGB. Results After RYGB, only patients with T2Dr had significantly increased REG1 alpha and REG3 gamma expression in the jejunum, as validated by quantitative RT-PCR. Conclusions Our data provide support for the hypothesis that increased jejunal expression of REG genes after RYGB affects T2Dr, possibly by playing an endocrine function. (AU)

• FAPESP's process: 13/03246-0 - Genomic, proteomic and metabolomic study of gastrointestinal tissue for patients with type 2 Diabetes mellitus after Roux-en-Y gastric bypass
• Grantee: Priscila Sala Kobal
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• FAPESP's process: 11/09612-3 - Changes in gene expression in gastric and intestinal tissue of type 2 Diabetes patients submitted to Roux-en-Y gastric bypass
• Grantee: Dan Linetzky Waitzberg
• Support Opportunities: Research Projects - Thematic Grants