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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Xyloglucan breakdown by endo-xyloglucanase family 74 from Aspergillus fumigatus

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Author(s):
de Lima Damasio, Andre Ricardo ; Rubio, Marcelo Ventura ; Goncalves, Thiago Augusto ; Persinoti, Gabriela Felix ; Segato, Fernando ; Prade, Rolf Alexander ; Contesini, Fabiano Jares ; de Souza, Amanda Pereira ; Buckeridge, Marcos Silveira ; Squina, Fabio Marcio
Total Authors: 10
Document type: Journal article
Source: Applied Microbiology and Biotechnology; v. 101, n. 7, p. 2893-2903, APR 2017.
Web of Science Citations: 7
Abstract

Xyloglucan is the most abundant hemicellulose in primary walls of spermatophytes except for grasses. Xyloglucan-degrading enzymes are important in lignocellulosic biomass hydrolysis because they remove xyloglucan, which is abundant in monocot-derived biomass. Fungal genomes encode numerous xyloglucanase genes, belonging to at least six glycoside hydrolase (GH) families. GH74 endo-xyloglucanases cleave xyloglucan backbones with unsubstituted glucose at the -1 subsite or prefer xylosyl-substituted residues in the -1 subsite. In this work, 137 GH74-related genes were detected by examining 293 Eurotiomycete genomes and Ascomycete fungi contained one or no GH74 xyloglucanase gene per genome. Another interesting feature is that the triad of tryptophan residues along the catalytic cleft was found to be widely conserved among Ascomycetes. The GH74 from Aspergillus fumigatus (AfXEG74) was chosen as an example to conduct comprehensive biochemical studies to determine the catalytic mechanism. AfXEG74 has no CBM and cleaves the xyloglucan backbone between the unsubstituted glucose and xylose-substituted glucose at specific positions, along the XX motif when linked to regions deprived of galactosyl branches. It resembles an endo-processive activity, which after initial random hydrolysis releases xyloglucan-oligosaccharides as major reaction products. This work provides insights on phylogenetic diversity and catalytic mechanism of GH74 xyloglucanases from Ascomycete fungi. (AU)

FAPESP's process: 14/18714-2 - Enzymatic oxidation of sugarcane bagasse: discovery, characterization and new application of oxidative enzymes active in carbohydrates, applied to the enhancement of a fungal cell factory
Grantee:Fernando Segato
Support type: Program for Research on Bioenergy (BIOEN) - Young Investigators Grants
FAPESP's process: 11/02169-7 - Aspergillus nidulans as a model for heterologous expression of cellulases and hemicellulases
Grantee:André Ricardo de Lima Damasio
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 12/20549-4 - Secretion of heterologous glycoproteins in Aspergillus: effect of glycosylation pattern in functional parameters of glycosyl hydrolases
Grantee:André Ricardo de Lima Damasio
Support type: Program for Research on Bioenergy (BIOEN) - Young Investigators Grants
FAPESP's process: 08/58037-9 - Library generation for biomass-conversion enzymes from soil metagenome
Grantee:Fábio Márcio Squina
Support type: Program for Research on Bioenergy (BIOEN) - Young Investigators Grants