Polyamines regulate phosphorylation-dephosphorylation kinetics in a crustacean gill (Na+, K+)-ATPase

Author(s)

Lucena, Malson Neilson ; Garcon, Daniela Pereira ; Leite Fontes, Carlos Frederico ; McNamara, John Campbell ; Leone, Francisco Assis

• Document type: Journal article
• Source: Molecular and Cellular Biochemistry; v. 429, n. 1-2, p. 187-198, MAY 2017.
• Web of Science Citations: 4

Abstract

Aiming to clarify the mechanism of inhibition of (Na+, K+)-ATPase activity by polyamines, we examined the effects of exogenous putrescine, spermidine, and spermine on the kinetic behavior of phosphoenzyme-linked partial reactions using a microsomal gill (Na+, K+)-ATPase from juvenile and adult M. amazonicum, a freshwater palaemonid shrimp. The time course of phosphointermediate formation is greater (0.089 +/- 0.006 s(-1)) in adults than in juveniles (0.053 +/- 0.003 s(-1)) for spermidine, but similar to juveniles (0.059 +/- 0.004 s(-1)) for putrescine. Maximum phosphointermediate formation for the (Na+, K+)-ATPase from juveniles decreased by 46% and 32% with spermidine and putrescine, respectively. In adults, maximum phosphointermediate levels decreased by 50% and 8%, respectively. For both spermidine and putrescine, dephosphorylation rates were higher for adults than for juveniles, and were higher than in controls without polyamines. Spermine had a negligible effect (<10%) on phosphorylation/dephosphorylation rates of both juvenile and adult enzymes. This is the first report on the effects of polyamines on phosphoenzyme-linked partial reactions in juvenile and adult M. amazonicum gill (Na+, K+)-ATPases. Our findings suggest that the phosphorylation/dephosphorylation steps of this gill enzyme may be regulated by polyamines during ontogenetic development. (AU)

• FAPESP's process: 13/24252-9 - The (Na+,K+)-ATPase in osmoregulatory capacity in crab Ucides cordatus: biochemical and molecular view
• Grantee: Malson Neilson de Lucena
• Support type: Scholarships in Brazil - Post-Doctorate
• FAPESP's process: 10/17534-0 - Role of (Na,K)-ATPase in osmoregulation and excretion of ammonium ions in euryhaline crustaceans: identification and influence of FXYD2 on the regulation of gill (Na,K)-ATPase of the crab Callinectes danae
• Grantee: Francisco de Assis Leone
• Support type: Regular Research Grants
• FAPESP's process: 10/06395-9 - Role of (Na,K)-ATPase in osmorregulation and excretion of ammonium ions in euryhaline crustaceans: identification and influence of FXYD2 on the regulation of gill (Na,K)-ATPase of the crab Callinectes danae
• Grantee: Daniela Pereira Garçon
• Support type: Scholarships in Brazil - Post-Doctorate