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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Knocking down amygdalar PTP1B in diet-induced obese rats improves insulin signaling/action, decreases adiposity and may alter anxiety behavior

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Author(s):
Mendes, Natalia Ferreira ; Castro, Gisele ; Guadagnini, Dioze ; Tobar, Natalia ; Cognuck, Susana Quiros ; Kagohara Elias, Lucila Leico ; Boer, Patricia Aline ; Prada, Patricia Oliveira
Total Authors: 8
Document type: Journal article
Source: METABOLISM-CLINICAL AND EXPERIMENTAL; v. 70, p. 1-11, MAY 2017.
Web of Science Citations: 7
Abstract

Objective. Protein tyrosine phosphatase 1B (PTP1B) has been extensively implicated in the regulation of body weight, food intake, and energy. expenditure. The role of PTP1B appears to be cell and brain region dependent. Results. Herein, we demonstrated that chronic high-fat feeding enhanced PTP1B expression in the central nucleus of the amygdala (CeA) of rats compared to rats on chow: Knocking down PTP1B with oligonucleotide antisense (ASO) decreased its expression and was sufficient to improve the anorexigenic effect of insulin through IR/Akt signaling in the CeA. ASO treatment reduces body weight, fat mass, serum leptin levels, and food intake and also increases energy expenditure, without altering ambulatory activity. These changes were explained, at least in part, by the improvement of insulin sensitivity in the CeA, decreasing NPY and enhancing oxytocin expression. There was a slight decline in fasting blood glucose and serum insulin levels possibly due to leanness in rats treated with ASO. Surprisingly, the elevated plus maze test revealed an anxiolytic behavior after reduction of PTP1B in the CeA. Conclusions. Thus, the present study highlights the deleterious role that the amygdalar PTP1B has on energy homeostasis in obesity states. The reduction of PTP1B in the CeA may be a strategy for the treatment of obesity, insulin resistance and anxiety disorders. (C) 2017 Elsevier Inc. All rights reserved. (AU)

FAPESP's process: 13/07607-8 - OCRC - Obesity and Comorbidities Research Center
Grantee:Licio Augusto Velloso
Support type: Research Grants - Research, Innovation and Dissemination Centers - RIDC
FAPESP's process: 15/00343-0 - The effect of obesity in the regulation of AMPK in the amygdala: implications on energy metabolism
Grantee:Patrícia de Oliveira Prada
Support type: Regular Research Grants