Defeating Bacterial Resistance and Preventing Mammalian Cells Toxicity Through Rational Design of Antibiotic-Functionalized Nanoparticles

Author(s)

Affonso de Oliveira, Jessica Fernanda ; Saito, Angela ; Bido, Ariadne Tuckmantel ; Kobarg, Joerg ; Stassen, Hubert Karl ; Cardoso, Mateus Borba

• Document type: Journal article
• Source: SCIENTIFIC REPORTS; v. 7, MAY 2 2017.
• Web of Science Citations: 18

Abstract

The rational synthesis of alternative materials is highly demanding due to the outbreak of infectious diseases and resistance to antibiotics. Herein, we report a tailored nanoantibiotic synthesis protocol where the antibiotic binding was optimized on the silver-silica core-shell nanoparticles surface to maximize biological responses. The obtained silver nanoparticles coated with mesoporous silica functionalized with ampicillin presented remarkable antimicrobial effects against susceptible and antibiotic-resistant Escherichia coli. In addition, these structures were not cell-death inducers and different steps of the mitotic cell cycle (prophase, anaphase and metaphase) were clearly identified. The superior biological results were attributed to a proper and tailored synthesis strategy. (AU)

• FAPESP's process: 14/22322-2 - Functionalization of silica nanoparticles: increasing biological interaction
• Grantee: Mateus Borba Cardoso
• Support Opportunities: Regular Research Grants
• FAPESP's process: 11/15937-2 - Funcionalization of silver nanoparticles with beta-lactam antibiotics: an alternative to bacterial resistance
• Grantee: Jessica Fernanda Affonso de Oliveira
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 15/25406-5 - Organizing matter: colloids formed by association of surfactants, polymers and nanoparticles
• Grantee: Watson Loh
• Support Opportunities: Research Projects - Thematic Grants