Identification of Corynebacterium bovis by MALDI-mass spectrometry

Author(s)

Langoni, Hello ; Camargo da Silva, Carolina Polo ; Troncarelli, Marcella Zampoli ; Tata, Alessandra ; Anacleto Belaz, Katia Roberta ; Eberlin, Marcos Nogueira ; Joaquim, Samea Fernandes ; Guimaraes, Felipe Freitas ; Pardo, Renata Bonini ; Gomes, Eduardo Nardini

• Document type: Journal article
• Source: JOURNAL OF DAIRY SCIENCE; v. 100, n. 6, p. 4287-4289, JUN 2017.
• Web of Science Citations: 0

Abstract

Corynebacterium bovis is a mastitis-causing microorganism responsible for economic losses related to decrease in milk production. The aim of the study was identify Corynebacterium, spp. strains recovered from milk samples of subclinical mastitis by using matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS). Samples were collected during a 10-mo mastitis-monitoring program in a high-production dairy farm. In this study, 80 strains were analyzed; from these 54 (67.5%) were identified at species level as Corynebacterium bovis, 24 (31.2%) isolates were identified at the genus level as Corynebacterium spp., and only 1 (1.35%) isolated had unreliable identification. Results demonstrated that MALDI-MS could be an important technique for the identification of Corynebacterium spp. in milk. (AU)

• FAPESP's process: 12/24213-0 - Characterization of Corynebacterium spp. isolates and its relationship with other mastitis pathogens; effects on somatic cell COUNT/ML and milk production
• Grantee: Carolina Polo Camargo da Silva
• Support Opportunities: Scholarships in Brazil - Scientific Initiation