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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Fatty Acid Binding Protein 3 And Transzonal Projections Are Involved In Lipid Accumulation During In Vitro Maturation Of Bovine Oocytes

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Author(s):
del Collado, Maite ; da Silveira, Juliano Coelho ; Sangalli, Juliano Rodrigues ; Andrade, Gabriella Mamede ; da Silva Sousa, Leticia Rabello ; Silva, Luciano Andrade ; Meirelles, Flavio Vieira ; Perecin, Felipe
Total Authors: 8
Document type: Journal article
Source: SCIENTIFIC REPORTS; v. 7, JUN 1 2017.
Web of Science Citations: 14
Abstract

Oocytes that undergo in vitro maturation (IVM) are metabolically abnormal and accumulate excess lipid content. However, the mechanism of lipid accumulation and the role of cumulus cells in this process are unclear. Recently, it was shown that fatty acid binding proteins (FABPs) performed intra- and extracellular fatty acid transport. We postulated that FABP3 might be responsible for fatty acid transport from cumulus cells to the oocytes via transzonal projections (TZPs) during IVM. Transcript and protein levels of FABP3 were analyzed in both in vivo- and in vitro-matured cumulus-oocyte-complexes and were increased in IVM samples. Further analysis showed increased lipid content in oocytes and cumulus cells in IVM samples compared to in vivo- derived. We therefore speculated that altered traffic of fatty acids via FABP3 during IVM was the mechanism leading to the excess of lipids accumulated within IVM oocytes. Furthermore, we demonstrated an increase in FABP3 levels and lipid content during the first 9 h of IVM, further strengthening the possibility of fatty acid transport via FABP3 and TZPs. Additionally, disruptions of TZPs during IVM decreased lipid accumulation in oocytes. Our results shed light on a possible mechanism involving FABP3 and TZPs that causes excess lipid accumulation in oocytes during IVM. (AU)

FAPESP's process: 14/22887-0 - Cell-secreted vesicles containing miRNAs modulate epigenetic changes during in vitro culture of bovine gametes and embryos
Grantee:Juliano Coelho da Silveira
Support type: Research Grants - Young Investigators Grants
FAPESP's process: 15/26056-8 - Protein quantification related to the lipid metabolism of cumulus cells during in vivo and in vitro maturation of bovines oocytes
Grantee:Letícia Rabello da Silva Sousa
Support type: Scholarships in Brazil - Scientific Initiation
FAPESP's process: 14/21034-3 - Intra and intercellular routes during foliculogenesis and in vivo and in vitro oocyte maturation
Grantee:Felipe Perecin
Support type: Regular Research Grants
FAPESP's process: 14/03281-3 - Lipid-mitochondrial dynamic and oxidative metabolism modulators in in vitro produced bovine embryos
Grantee:Maite Del Collado Barrondo
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 13/08135-2 - CTC - Center for Cell-Based Therapy
Grantee:Dimas Tadeu Covas
Support type: Research Grants - Research, Innovation and Dissemination Centers - RIDC