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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Prevalence of gyrA Mutations in Nalidixic Acid-Resistant Strains of Salmonella Enteritidis Isolated from Humans, Food, Chickens, and the Farm Environment in Brazil

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Author(s):
Campioni, Fabio ; Souza, Roberto Antonio ; Martins, Vinicius Vicente ; Stehling, Eliana Guedes ; Morato Bergamini, Alzira Maria ; Falcao, Juliana Pfrimer
Total Authors: 6
Document type: Journal article
Source: MICROBIAL DRUG RESISTANCE; v. 23, n. 4, p. 421-428, JUN 2017.
Web of Science Citations: 7
Abstract

Salmonella Enteritidis strains that are resistant to nalidixic acid and exhibit reduced susceptibility to fluoroquinolones have been increasing worldwide. In Brazil, few studies have been conducted to elucidate the quinolone resistance mechanisms of S. Enteritidis strains. This study analyzed the profile of gyrA, gyrB, parC, and parE mutations and plasmid-mediated quinolone resistance (PMQR) mechanisms in S. Enteritidis Nal(R) strains isolated in Brazil. Moreover, the minimum inhibitory concentrations (MICs) of ciprofloxacin were evaluated in 84 Nal(R) strains and compared with 20 Nal(S) strains. The mutation profiles of the gyrA gene were accessed by high-resolution melting analysis and gyrB, parC, and parE by quinolone resistance-determining region sequencing. The MICs of ciprofloxacin were accessed with Etest (R). The strains were divided into five gyrA melting profiles. The Nal(R) strains exhibited the following amino acid substitutions: Ser97 -> Pro, Ser83 -> Phe, Asp87 -> Asn, or Asp87 -> Tyr. The average MICs of ciprofloxacin was 0.006 mu g/ml in the Nal(S) and 0.09 mu g/ml in the Nal(R) strains. No points of mutation were observed in the genes gyrB, parC, and parE. The qnrB gene was found in two strains. In conclusion, the reduced susceptibility to ciprofloxacin observed in Nal(R) strains may cause treatment failures once this drug is commonly used to treat Salmonella infections. Moreover, this reduced susceptibility in these Brazilian strains was provided by target alteration of gene gyrA and not by mobile elements, such as resistance plasmids. (AU)

FAPESP's process: 09/09998-9 - Molecular Typing and analysis of the genetic diversity of Salmonella Enteritidis strains isolated from humans and food in São Paulo State, Brazil.
Grantee:Fábio Campioni
Support Opportunities: Scholarships in Brazil - Doctorate