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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Urinary Tamm-Horsfall protein, albumin, vitamin D-binding protein, and retinol-binding protein as early biomarkers of chronic kidney disease in dogs

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Author(s):
Chacar, Fernanda ; Kogika, Marcia ; Sanches, Talita R. ; Caragelasco, Douglas ; Martorelli, Cinthia ; Rodrigues, Camila ; Capcha, Jose Manuel C. ; Chew, Dennis ; Andrade, Lucia
Total Authors: 9
Document type: Journal article
Source: PHYSIOLOGICAL REPORTS; v. 5, n. 11 JUN 2017.
Web of Science Citations: 3
Abstract

Proteinuria is a marker and mediator of chronic kidney disease (CKD). In clinical practice, the urinary protein-to-creatinine ratio (UP/C) is of limited usefulness, because it indicates only the magnitude of proteinuria and not the origin of the loss (glomerular or tubular). The complete assessment of proteinuria includes quantitative and qualitative evaluations, both of which are required in order to optimize the therapy. In addition to measuring the UP/C, we performed SDS-PAGE and western blotting to determine the expression of albumin, vitamin D-binding protein (VDBP), retinol-binding protein (RBP), and Tamm-Horsfall protein (THP) in urine samples of 49 dogs: healthy (control) dogs (n=9); and dogs with CKD (n=40), stratified by stage. In the dogs with stage 3 or 4 CKD, there was a predominance of tubular proteins. Neither VDBP nor RBP was observed in the urine of the control dogs. Among the dogs with stage 1 or 2 CKD, VDBP and RBP were detected in those without proteinuria or with borderline proteinuria. The expression of urinary albumin was significantly higher in the stage 4 group than in any other group (P <= 0.01). In the stage 4 group, urinary THP was either undetectable or lower than in the control group (P <= 0.01). In conclusion, urinary VDBP and RBP might act as early markers of kidney injury, and a decrease in urinary THP could be an indicator of CKD progression. (AU)

FAPESP's process: 10/19012-0 - Evaluation of hematopoietic stem cell treatment in dogs with chronic renal failure
Grantee:Lucia da Conceição Andrade
Support Opportunities: Research Projects - Thematic Grants