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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Schistosoma mansoni displays an adenine phosphoribosyltransferase preferentially expressed in mature female gonads and vitelaria

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Author(s):
Zeraik, Ana Eliza ; Balasco Serrao, Vitor Hugo ; Romanello, Larissa ; Torini, Juliana Roberta ; Cassago, Alexandre ; DeMarco, Ricardo ; Pereira, Humberto D'Muniz
Total Authors: 7
Document type: Journal article
Source: Molecular and Biochemical Parasitology; v. 214, p. 82-86, JUN 2017.
Web of Science Citations: 6
Abstract

Schistosoma mansoni depends upon the purine salvage pathway to obtain purine nucleotides; therefore, enzymes from this pathway are essential for parasite survival. Here, we focused on the adenine phosphoribosyltransferase (APRT) enzyme, which catalyzes the condensation reaction between adenine and PRPP (5-phosphoribosylpyrophosphate) to produce AMP and PPi. Kinetic experiments using the heterologously expressed protein of one APRT isoform from S. mansoni indicate that it is catalytically active, and whole-mount in situ hybridization studies indicate that the transcripts of this protein are concentrated in the posterior region of the ovary and vitellaria of female adult worms. Moreover, a phylogenetic analysis has shown that APRT exists in multiple copies originating from gene duplications at the base of the Schistosoma genus. Other enzymes from the purine and pyrimidine salvage pathways have also been found to present multiple copies in schistosomes, suggesting that evolutionary pressure to diversify these genes' families may be related to a specialized role in parasite reproduction. (AU)

FAPESP's process: 12/10213-9 - Structural and functional studies of Purine nucleoside phosphorylase isoform 2, Methylthioadenosine phosphorylase and participants enzymes of GTP production from purine salvage pathway from Schistosoma mansoni.
Grantee:Juliana Roberta Torini de Souza Oliveira
Support Opportunities: Scholarships in Brazil - Doctorate
FAPESP's process: 12/05532-8 - Structural and kinetic studies of adenosine kinase, hypoxanthine-guanine phosphoribosyltransferase, Adenilsuccinate Synthetase and Adenilsuccinato Lyase enzymes from Schistosoma mansoni
Grantee:Larissa Romanello
Support Opportunities: Scholarships in Brazil - Doctorate
FAPESP's process: 12/14223-9 - Structural and kinetical studies of the enzymes involved in the purine metabolism in Schistosoma mansoni
Grantee:Humberto D'Muniz Pereira
Support Opportunities: Regular Research Grants
FAPESP's process: 13/20715-4 - Studies of the correlation between septin dynamics and Ca2+ homeostasis in muscular cells from Schistosoma mansoni
Grantee:Ana Eliza Zeraik
Support Opportunities: Scholarships in Brazil - Post-Doctoral
FAPESP's process: 12/23730-1 - Characterization of macromolecular interactions of proteins involved in the selenocysteine synthesis from Escherichia coli
Grantee:Vitor Hugo Balasco Serrão
Support Opportunities: Scholarships in Brazil - Doctorate