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(Reference retrieved automatically from SciELO through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Copaiba Oil and Its Constituent Copalic Acid as Chemotherapeutic Agents against Dermatophytes

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Author(s):
Marcela T. Nakamura [1] ; Eliana H. Endo [2] ; João Paulo B. de Sousa [3] ; Daniel R. Callejon [4] ; Tânia Ueda-Nakamura [5] ; Benedito P. Dias Filho [6] ; Osvaldo de Freitas [7] ; Celso V. Nakamura [8] ; Norberto P. Lopes [9]
Total Authors: 9
Affiliation:
[1] Universidade de São Paulo. Faculdade Ciências Farmacêuticas de Ribeirão Preto - Brasil
[2] Universidade Estadual de Maringá. Laboratório de Inovação Tecnológica no Desenvolvimento de Fármacos e Cosméticos. Departamento de Ciências Básicas da Saúde - Brasil
[3] Universidade de São Paulo. Faculdade Ciências Farmacêuticas de Ribeirão Preto - Brasil
[4] Universidade de São Paulo. Faculdade Ciências Farmacêuticas de Ribeirão Preto - Brasil
[5] Universidade Estadual de Maringá. Laboratório de Inovação Tecnológica no Desenvolvimento de Fármacos e Cosméticos. Departamento de Ciências Básicas da Saúde - Brasil
[6] Universidade Estadual de Maringá. Laboratório de Inovação Tecnológica no Desenvolvimento de Fármacos e Cosméticos. Departamento de Ciências Básicas da Saúde - Brasil
[7] Universidade de São Paulo. Faculdade Ciências Farmacêuticas de Ribeirão Preto - Brasil
[8] Universidade Estadual de Maringá. Laboratório de Inovação Tecnológica no Desenvolvimento de Fármacos e Cosméticos. Departamento de Ciências Básicas da Saúde - Brasil
[9] Universidade de São Paulo. Faculdade Ciências Farmacêuticas de Ribeirão Preto - Brasil
Total Affiliations: 9
Document type: Journal article
Source: Journal of the Brazilian Chemical Society; v. 28, n. 8, p. 1377-1383, 2017-08-00.
Abstract

Copaiba oil, an oleoresin extracted from Copaifera genus, has been widely used in popular medicine for the treatment of several diseases. The aim of this study was to investigate the antifungal activity of the copaiba oil and its isolated compounds caryophyllene oxide, copalic acid and acetoxycopalic acid against Trichophyton rubrum, Trichophyton mentagrophytes and Microsporum gypseum strains, using microdilution method and microscopy techniques. It was found that the copaiba oil and the copalic acid were active against dermatophytes by minimal inhibitory concentration (MIC) and minimal fungicidal concentration (MFC) tests. The MIC and MFC of copaiba oil against T. rubrum, T. mentagrophytes and M. gypseum were 125 µg mL-1 (250 µg mL-1), 500 µg mL-1 (500 µg mL-1) and 250 µg mL-1 (250 µg mL-1), respectively. For copalic acid, the MIC and MFC were 50 µg mL-1 (100 µg mL-1), 100 µg mL-1 (100 µg mL-1) and 50 µg mL-1 (100 µg mL-1), respectively. Fluorescence microscopy and scanning electronic microscopy were used to investigate inhibition on hyphal growth by compounds, copaiba oil and copalic acid, showing a strong inhibition and an irregular growth pattern. Cell wall, cytoplasmic membrane and intracellular contents were also damaged. In conclusion, copaiba oil and copalic acid showed great activity against dermatophytes, being potential compounds for the development of antifungal drugs. (AU)

FAPESP's process: 14/50265-3 - Distribution and metabolism of natural and synthetic xenobiotics: from the comprehension of reactional process to tissue imaging generation
Grantee:Norberto Peporine Lopes
Support Opportunities: BIOTA-FAPESP Program - Thematic Grants