Cholecalciferol decreases inflammation and improves vitamin D regulatory enzymes in lymphocytes in the uremic environment: A randomized controlled pilot trial

Author(s)

Carvalho, Jose Tarcisio G. ; Schneider, Marion ; Cuppari, Lilian ; Grabulosa, Caren C. ; Aoike, Danilo T. ; Redublo, Beata Marie Q. ; Batista, Marcelo C. ; Cendoroglo, Miguel ; Moyses, Rosa Maria ; Dalboni, Maria Aparecida

• Document type: Journal article
• Source: PLoS One; v. 12, n. 6 JUN 30 2017.
• Web of Science Citations: 10

Abstract

It has been reported that vitamin D regulates the immune system. However, whether vitamin D repletion modulates inflammatory responses in lymphocytes from dialysis patients is unclear. In the clinical trial, thirty-two (32) dialysis patients with 25 vitamin D <= 20ng/mL were randomized to receive either supplementation of cholecalciferol 100,000 UI/week/3 months (16 patients) or placebo (16 patients). In the in vitro study, B and T lymphocytes from 12 healthy volunteers (HV) were incubated with or without uremic serum in the presence or absence of 25 or 1,25 vitamin D. We evaluated the intracellular expression of IL-6, IFN-. TLR7, TLR9, VDR, CYP27b1 and CYP24a1 by flow cytometry. We observed a reduction in the expression of TLR7, TLR9, INF-gamma and CYP24a1 and an increase in VDR and CYP27b1 expression in patients which were supplemented with cholecalciferol, whereas no differences were found in the placebo group. Uremic serum increased the intracellular expression of IL-6, IFN-gamma, TLR7, TLR9, VDR, CYP27b1 and CYP24a1. Treatment with 25 or 1,25 vitamin D decreased IL-6 and TLR9. CYP24a1 silencing plus treatment with 25 and/or 1,25 vitamin D had an additional reduction effect on IL-6, IFN-gamma, TLR7 and TLR9 expression. This is the first study showing that cholecalciferol repletion has an anti-inflammatory effect and improves vitamin D intracellular regulatory enzymes on lymphocytes from dialysis patients. (AU)

• FAPESP's process: 12/21345-3 - Effects of cholecalciferol on modulation of expression of toll-like receptors (TLR-7 e TLR-9) and IL-6 e INF-gamma production in the b cells in uremia
• Grantee: Miguel Cendoroglo Neto
• Support Opportunities: Regular Research Grants
• FAPESP's process: 12/19314-2 - Effect of cholecalciferol on TLR-7 and TLR-9 expression and IL-6 and INF-y production by Lymphocytes B in uremia
• Grantee: José Tarcísio Giffoni de Carvalho Júnior
• Support Opportunities: Scholarships in Brazil - Doctorate